| Parameters | Input values |
|---|---|
| Folder name : | Example data |
| Sequence name : | CDH1 |
| Group : | High methylated DNA |
| Experiment type : | Direct-BSP |
| Replicate number : | 3 |
| Date of sequencing #1 : | 2022-05-02 |
| Date of sequencing #2 : | 2022-05-02 |
| Date of analysis : | 2023-06-09 17:23 |
| Prefix of output files : | CDH1_High methylated DNA_3 |
| Reference DNA sequence information | Input values |
|---|---|
| Name | CDH1 |
| Strand used for primer design | Plus strand |
| Length | 341 pb |
| Coordinates on reference genome | chr16:68770940-68771280 |
## CCACCGGCGGGGCTGGGATTCGAACCCAGTGGAATCAGAACCGTGCAGGTCCCATAACCCACCTAGACCCTAGCAACTCCAGGCTAGAGGGTCACCGCGTCTATGCGAGGCCGGGTGGGCGGGCCGTCAGCTCCGCCCTGGGGAGGGGTCCGCGCTGCTGATTGGCTGTGGCCGGCAGGTGAACCCTCAGCCAATCAGCGGTACGGGGGGCGGTGCCTCCGGGGCTCACCTGGCTGCAGCCACGCACCCCCTCTCAGTGGCGTCGGAACTGCAAAGCACCTGTGAGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGC
## GCGGTCGGGTCGGGCCGGGCTGGAGCGGGCTGGAGTCTGAACTGACTTCCGCAAGCTCACAGGTGCTTTGCAGTTCCGACGCCACTGAGAGGGGGTGCGTGGCTGCAGCCAGGTGAGCCCCGGAGGCACCGCCCCCCGTACCGCTGATTGGCTGAGGGTTCACCTGCCGGCCACAGCCAATCAGCAGCGCGGACCCCTCCCCAGGGCGGAGCTGACGGCCCGCCCACCCGGCCTCGCATAGACGCGGTGACCCTCTAGCCTGGAGTTGCTAGGGTCTAGGTGGGTTATGGGACCTGCACGGTTCTGATTCCACTGGGTTCGAATCCCAGCCCCGCCGGTGG
| CG number | chr | start | end | C position | G position |
|---|---|---|---|---|---|
| 1 | chr16 | 68770944 | 68770945 | 5 | 6 |
| 2 | chr16 | 68770947 | 68770948 | 8 | 9 |
| 3 | chr16 | 68770960 | 68770961 | 21 | 22 |
| 4 | chr16 | 68770981 | 68770982 | 42 | 43 |
| 5 | chr16 | 68771035 | 68771036 | 96 | 97 |
| 6 | chr16 | 68771037 | 68771038 | 98 | 99 |
| 7 | chr16 | 68771045 | 68771046 | 106 | 107 |
| 8 | chr16 | 68771051 | 68771052 | 112 | 113 |
| 9 | chr16 | 68771059 | 68771060 | 120 | 121 |
| 10 | chr16 | 68771064 | 68771065 | 125 | 126 |
| 11 | chr16 | 68771073 | 68771074 | 134 | 135 |
| 12 | chr16 | 68771090 | 68771091 | 151 | 152 |
| 13 | chr16 | 68771092 | 68771093 | 153 | 154 |
| 14 | chr16 | 68771112 | 68771113 | 173 | 174 |
| 15 | chr16 | 68771138 | 68771139 | 199 | 200 |
| 16 | chr16 | 68771143 | 68771144 | 204 | 205 |
| 17 | chr16 | 68771150 | 68771151 | 211 | 212 |
| 18 | chr16 | 68771159 | 68771160 | 220 | 221 |
| 19 | chr16 | 68771182 | 68771183 | 243 | 244 |
| 20 | chr16 | 68771200 | 68771201 | 261 | 262 |
| 21 | chr16 | 68771203 | 68771204 | 264 | 265 |
| 22 | chr16 | 68771230 | 68771231 | 291 | 292 |
| 23 | chr16 | 68771254 | 68771255 | 315 | 316 |
| 24 | chr16 | 68771264 | 68771265 | 325 | 326 |
| 25 | chr16 | 68771269 | 68771270 | 330 | 331 |
| 26 | chr16 | 68771274 | 68771275 | 335 | 336 |
| 27 | chr16 | 68771278 | 68771279 | 339 | 340 |
| CG number | chr | start | end | C position | G position |
|---|---|---|---|---|---|
| 27 | chr16 | 68771278 | 68771279 | 2 | 3 |
| 26 | chr16 | 68771274 | 68771275 | 6 | 7 |
| 25 | chr16 | 68771269 | 68771270 | 11 | 12 |
| 24 | chr16 | 68771264 | 68771265 | 16 | 17 |
| 23 | chr16 | 68771254 | 68771255 | 26 | 27 |
| 22 | chr16 | 68771230 | 68771231 | 50 | 51 |
| 21 | chr16 | 68771203 | 68771204 | 77 | 78 |
| 20 | chr16 | 68771200 | 68771201 | 80 | 81 |
| 19 | chr16 | 68771182 | 68771183 | 98 | 99 |
| 18 | chr16 | 68771159 | 68771160 | 121 | 122 |
| 17 | chr16 | 68771150 | 68771151 | 130 | 131 |
| 16 | chr16 | 68771143 | 68771144 | 137 | 138 |
| 15 | chr16 | 68771138 | 68771139 | 142 | 143 |
| 14 | chr16 | 68771112 | 68771113 | 168 | 169 |
| 13 | chr16 | 68771092 | 68771093 | 188 | 189 |
| 12 | chr16 | 68771090 | 68771091 | 190 | 191 |
| 11 | chr16 | 68771073 | 68771074 | 207 | 208 |
| 10 | chr16 | 68771064 | 68771065 | 216 | 217 |
| 9 | chr16 | 68771059 | 68771060 | 221 | 222 |
| 8 | chr16 | 68771051 | 68771052 | 229 | 230 |
| 7 | chr16 | 68771045 | 68771046 | 235 | 236 |
| 6 | chr16 | 68771037 | 68771038 | 243 | 244 |
| 5 | chr16 | 68771035 | 68771036 | 245 | 246 |
| 4 | chr16 | 68770981 | 68770982 | 299 | 300 |
| 3 | chr16 | 68770960 | 68770961 | 320 | 321 |
| 2 | chr16 | 68770947 | 68770948 | 333 | 334 |
| 1 | chr16 | 68770944 | 68770945 | 336 | 337 |
Bisulfite converted sequence of the plus strand as indicated as the template strand.
## TTATCGGCGGGGTTGGGATTCGAATTTAGTGGAATTAGAATCGTGTAGGTTTTATAATTTATTTAGATTTTAGTAATTTTAGGTTAGAGGGTTATCGCGTTTATGCGAGGTCGGGTGGGCGGGTCGTTAGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTTGCGGAAGTTAGTTTAGATTTTAGTTCGTTTTAGTTCGGTTCGATTCGATCGT
Reverse complement of the bisulfite converted sequence from the plus strand.
## ACGATCGAATCGAACCGAACTAAAACGAACTAAAATCTAAACTAACTTCCGCAAACTCACAAATACTTTACAATTCCGACGCCACTAAAAAAAAATACGTAACTACAACCAAATAAACCCCGAAAACACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAAATCTAAATAAATTATAAAACCTACACGATTCTAATTCCACTAAATTCGAATCCCAACCCCGCCGATAA
| Thresholds | Values |
|---|---|
| Minimum length of the trimmed sequence : | 30 bp |
| Minimum Phred quality score : | 30 |
| Minimum ratio of primary peak : | 0.75 |
| Minimum percentage of non-mixed positions : | 75 % |
| Sequencing | Length | Start position | End position | Mean of Phred quality scores | Mean of primary peak ratio | Percentage of non-mixed positions | Trimmed sequence quality |
|---|---|---|---|---|---|---|---|
| #1 | 205 | 27 | 231 | 46.68 | 0.94 | 89.27 | Correct |
| #2 | 207 | 7 | 213 | 42.54 | 0.96 | 94.20 | Correct |
Sequence of primary peaks:
Length: 244 bp
## TAATAAGGGGTAATTATGGGTTGAGGGTTTTGCGTTGTGCGAAGGGCGGGTGGGCGGGCCGTTGGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTTCCTCGACTGTGCCTTCTAAA
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 3 | 61 | 0 | 0 | 64 | 0.05 | 0.95 | 0.00 | 0.00 | T | 13 | 0.95 |
| 2 | 7 | 0 | 0 | 0 | 7 | 1.00 | 0.00 | 0.00 | 0.00 | A | 14 | 1.00 |
| 3 | 19 | 0 | 0 | 0 | 19 | 1.00 | 0.00 | 0.00 | 0.00 | A | 10 | 1.00 |
| 4 | 0 | 29 | 0 | 0 | 29 | 0.00 | 1.00 | 0.00 | 0.00 | T | 13 | 1.00 |
| 5 | 14 | 0 | 0 | 0 | 14 | 1.00 | 0.00 | 0.00 | 0.00 | A | 13 | 1.00 |
| 6 | 14 | 0 | 0 | 0 | 14 | 1.00 | 0.00 | 0.00 | 0.00 | A | 8 | 1.00 |
| 7 | 0 | 0 | 176 | 0 | 176 | 0.00 | 0.00 | 1.00 | 0.00 | G | 10 | 1.00 |
| 8 | 0 | 0 | 211 | 0 | 211 | 0.00 | 0.00 | 1.00 | 0.00 | G | 14 | 1.00 |
| 9 | 0 | 0 | 132 | 0 | 132 | 0.00 | 0.00 | 1.00 | 0.00 | G | 9 | 1.00 |
| 10 | 0 | 0 | 113 | 0 | 113 | 0.00 | 0.00 | 1.00 | 0.00 | G | 14 | 1.00 |
| 11 | 0 | 17 | 0 | 0 | 17 | 0.00 | 1.00 | 0.00 | 0.00 | T | 3 | 1.00 |
| 12 | 2249 | 0 | 776 | 0 | 3025 | 0.74 | 0.00 | 0.26 | 0.00 | A | 8 | 0.74 |
| 13 | 4167 | 0 | 0 | 0 | 4167 | 1.00 | 0.00 | 0.00 | 0.00 | A | 8 | 1.00 |
| 14 | 4791 | 12799 | 0 | 0 | 17590 | 0.27 | 0.73 | 0.00 | 0.00 | T | 15 | 0.73 |
| 15 | 0 | 12600 | 0 | 0 | 12600 | 0.00 | 1.00 | 0.00 | 0.00 | T | 17 | 1.00 |
| 16 | 14 | 0 | 0 | 0 | 14 | 1.00 | 0.00 | 0.00 | 0.00 | A | 22 | 1.00 |
| 17 | 0 | 9598 | 0 | 0 | 9598 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 18 | 0 | 0 | 3 | 0 | 3 | 0.00 | 0.00 | 1.00 | 0.00 | G | 12 | 1.00 |
| 19 | 0 | 0 | 4132 | 0 | 4132 | 0.00 | 0.00 | 1.00 | 0.00 | G | 20 | 1.00 |
| 20 | 4 | 5 | 4779 | 0 | 4788 | 0.00 | 0.00 | 1.00 | 0.00 | G | 22 | 1.00 |
| 21 | 0 | 4608 | 0 | 0 | 4608 | 0.00 | 1.00 | 0.00 | 0.00 | T | 16 | 1.00 |
| 22 | 2759 | 4002 | 0 | 0 | 6761 | 0.41 | 0.59 | 0.00 | 0.00 | T | 5 | 0.59 |
| 23 | 0 | 0 | 946 | 0 | 946 | 0.00 | 0.00 | 1.00 | 0.00 | G | 5 | 1.00 |
| 24 | 3989 | 0 | 0 | 0 | 3989 | 1.00 | 0.00 | 0.00 | 0.00 | A | 20 | 1.00 |
| 25 | 0 | 0 | 3676 | 0 | 3676 | 0.00 | 0.00 | 1.00 | 0.00 | G | 30 | 1.00 |
| 26 | 6 | 0 | 5006 | 0 | 5012 | 0.00 | 0.00 | 1.00 | 0.00 | G | 30 | 1.00 |
| 27 | 0 | 2 | 2368 | 0 | 2370 | 0.00 | 0.00 | 1.00 | 0.00 | G | 33 | 1.00 |
| 28 | 2 | 2342 | 2 | 0 | 2346 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 29 | 1619 | 1818 | 0 | 0 | 3437 | 0.47 | 0.53 | 0.00 | 0.00 | T | 17 | 0.53 |
| 30 | 0 | 2202 | 0 | 0 | 2202 | 0.00 | 1.00 | 0.00 | 0.00 | T | 17 | 1.00 |
| 31 | 3 | 1323 | 0 | 580 | 1906 | 0.00 | 0.69 | 0.00 | 0.30 | T | 16 | 0.69 |
| 32 | 0 | 0 | 2518 | 0 | 2518 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 33 | 0 | 0 | 0 | 627 | 627 | 0.00 | 0.00 | 0.00 | 1.00 | C | 34 | 1.00 |
| 34 | 0 | 0 | 931 | 0 | 931 | 0.00 | 0.00 | 1.00 | 0.00 | G | 35 | 1.00 |
| 35 | 0 | 1617 | 0 | 0 | 1617 | 0.00 | 1.00 | 0.00 | 0.00 | T | 34 | 1.00 |
| 36 | 0 | 1901 | 3 | 0 | 1904 | 0.00 | 1.00 | 0.00 | 0.00 | T | 29 | 1.00 |
| 37 | 1579 | 0 | 2296 | 0 | 3875 | 0.41 | 0.00 | 0.59 | 0.00 | G | 24 | 0.59 |
| 38 | 0 | 1177 | 0 | 0 | 1177 | 0.00 | 1.00 | 0.00 | 0.00 | T | 27 | 1.00 |
| 39 | 0 | 1 | 796 | 0 | 797 | 0.00 | 0.00 | 1.00 | 0.00 | G | 16 | 1.00 |
| 40 | 610 | 0 | 0 | 707 | 1317 | 0.46 | 0.00 | 0.00 | 0.54 | C | 16 | 0.54 |
| 41 | 0 | 0 | 786 | 1 | 787 | 0.00 | 0.00 | 1.00 | 0.00 | G | 27 | 1.00 |
| 42 | 712 | 192 | 0 | 0 | 904 | 0.79 | 0.21 | 0.00 | 0.00 | A | 22 | 0.79 |
| 43 | 497 | 0 | 364 | 0 | 861 | 0.58 | 0.00 | 0.42 | 0.00 | A | 14 | 0.58 |
| 44 | 0 | 607 | 871 | 0 | 1478 | 0.00 | 0.41 | 0.59 | 0.00 | G | 12 | 0.59 |
| 45 | 0 | 0 | 2 | 0 | 2 | 0.00 | 0.00 | 1.00 | 0.00 | G | 21 | 1.00 |
| 46 | 0 | 0 | 2 | 0 | 2 | 0.00 | 0.00 | 1.00 | 0.00 | G | 18 | 1.00 |
| 47 | 0 | 349 | 1 | 860 | 1210 | 0.00 | 0.29 | 0.00 | 0.71 | C | 19 | 0.71 |
| 48 | 0 | 2 | 979 | 2 | 983 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 49 | 0 | 130 | 715 | 0 | 845 | 0.00 | 0.15 | 0.85 | 0.00 | G | 35 | 0.85 |
| 50 | 0 | 0 | 757 | 0 | 757 | 0.00 | 0.00 | 1.00 | 0.00 | G | 29 | 1.00 |
| 51 | 0 | 428 | 0 | 0 | 428 | 0.00 | 1.00 | 0.00 | 0.00 | T | 27 | 1.00 |
| 52 | 202 | 0 | 797 | 0 | 999 | 0.20 | 0.00 | 0.80 | 0.00 | G | 26 | 0.80 |
| 53 | 0 | 0 | 696 | 0 | 696 | 0.00 | 0.00 | 1.00 | 0.00 | G | 36 | 1.00 |
| 54 | 0 | 172 | 388 | 0 | 560 | 0.00 | 0.31 | 0.69 | 0.00 | G | 33 | 0.69 |
| 55 | 0 | 129 | 0 | 664 | 793 | 0.00 | 0.16 | 0.00 | 0.84 | C | 36 | 0.84 |
| 56 | 0 | 0 | 730 | 282 | 1012 | 0.00 | 0.00 | 0.72 | 0.28 | G | 25 | 0.72 |
| 57 | 0 | 1 | 741 | 349 | 1091 | 0.00 | 0.00 | 0.68 | 0.32 | G | 15 | 0.68 |
| 58 | 0 | 285 | 304 | 0 | 589 | 0.00 | 0.48 | 0.52 | 0.00 | G | 14 | 0.52 |
| 59 | 0 | 462 | 1 | 467 | 930 | 0.00 | 0.50 | 0.00 | 0.50 | C | 14 | 0.50 |
| 60 | 1 | 0 | 0 | 981 | 982 | 0.00 | 0.00 | 0.00 | 1.00 | C | 26 | 1.00 |
| 61 | 192 | 0 | 489 | 0 | 681 | 0.28 | 0.00 | 0.72 | 0.00 | G | 26 | 0.72 |
| 62 | 0 | 302 | 1 | 148 | 451 | 0.00 | 0.67 | 0.00 | 0.33 | T | 15 | 0.67 |
| 63 | 0 | 1241 | 1 | 1 | 1243 | 0.00 | 1.00 | 0.00 | 0.00 | T | 26 | 1.00 |
| 64 | 444 | 0 | 542 | 0 | 986 | 0.45 | 0.00 | 0.55 | 0.00 | G | 15 | 0.55 |
| 65 | 0 | 187 | 658 | 0 | 845 | 0.00 | 0.22 | 0.78 | 0.00 | G | 25 | 0.78 |
| 66 | 0 | 483 | 226 | 0 | 709 | 0.00 | 0.68 | 0.32 | 0.00 | T | 16 | 0.68 |
| 67 | 0 | 994 | 2 | 337 | 1333 | 0.00 | 0.75 | 0.00 | 0.25 | T | 16 | 0.75 |
| 68 | 0 | 772 | 0 | 0 | 772 | 0.00 | 1.00 | 0.00 | 0.00 | T | 40 | 1.00 |
| 69 | 0 | 416 | 0 | 994 | 1410 | 0.00 | 0.30 | 0.00 | 0.70 | C | 29 | 0.70 |
| 70 | 0 | 0 | 327 | 2 | 329 | 0.00 | 0.00 | 0.99 | 0.01 | G | 25 | 0.99 |
| 71 | 0 | 422 | 0 | 308 | 730 | 0.00 | 0.58 | 0.00 | 0.42 | T | 16 | 0.58 |
| 72 | 1 | 1294 | 0 | 0 | 1295 | 0.00 | 1.00 | 0.00 | 0.00 | T | 26 | 1.00 |
| 73 | 589 | 703 | 0 | 0 | 1292 | 0.46 | 0.54 | 0.00 | 0.00 | T | 15 | 0.54 |
| 74 | 622 | 921 | 1 | 0 | 1544 | 0.40 | 0.60 | 0.00 | 0.00 | T | 15 | 0.60 |
| 75 | 0 | 3 | 1242 | 0 | 1245 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 76 | 46 | 0 | 404 | 0 | 450 | 0.10 | 0.00 | 0.90 | 0.00 | G | 51 | 0.90 |
| 77 | 0 | 0 | 630 | 0 | 630 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 78 | 2 | 0 | 644 | 0 | 646 | 0.00 | 0.00 | 1.00 | 0.00 | G | 29 | 1.00 |
| 79 | 893 | 0 | 0 | 0 | 893 | 1.00 | 0.00 | 0.00 | 0.00 | A | 29 | 1.00 |
| 80 | 1 | 0 | 668 | 0 | 669 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 81 | 1 | 0 | 970 | 0 | 971 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 82 | 0 | 0 | 798 | 0 | 798 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 83 | 0 | 0 | 726 | 0 | 726 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 84 | 0 | 756 | 0 | 0 | 756 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 85 | 0 | 914 | 0 | 0 | 914 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 86 | 0 | 3 | 2 | 1071 | 1076 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 87 | 0 | 0 | 652 | 0 | 652 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 88 | 0 | 0 | 4 | 807 | 811 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 89 | 0 | 0 | 1138 | 0 | 1138 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 90 | 0 | 799 | 3 | 0 | 802 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 91 | 0 | 854 | 0 | 0 | 854 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 92 | 0 | 3 | 1047 | 0 | 1050 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 93 | 0 | 605 | 2 | 0 | 607 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 94 | 0 | 912 | 2 | 0 | 914 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 95 | 0 | 2 | 1004 | 0 | 1006 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 96 | 842 | 1 | 2 | 0 | 845 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 97 | 1 | 696 | 0 | 0 | 697 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 98 | 0 | 958 | 1 | 0 | 959 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 99 | 0 | 4 | 1226 | 0 | 1230 | 0.00 | 0.00 | 1.00 | 0.00 | G | 41 | 1.00 |
| 100 | 0 | 2 | 565 | 0 | 567 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 101 | 0 | 518 | 0 | 0 | 518 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 102 | 0 | 957 | 3 | 0 | 960 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 103 | 0 | 5 | 1344 | 0 | 1349 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 104 | 0 | 530 | 0 | 0 | 530 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 105 | 0 | 2 | 1294 | 0 | 1296 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 106 | 0 | 2 | 675 | 0 | 677 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 107 | 0 | 479 | 0 | 1 | 480 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 108 | 0 | 2 | 0 | 991 | 993 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 109 | 0 | 0 | 659 | 2 | 661 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 110 | 0 | 0 | 757 | 0 | 757 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 111 | 2 | 421 | 1 | 0 | 424 | 0.00 | 0.99 | 0.00 | 0.00 | T | 51 | 0.99 |
| 112 | 733 | 1 | 1 | 0 | 735 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 113 | 0 | 0 | 435 | 0 | 435 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 114 | 0 | 2 | 845 | 0 | 847 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 115 | 0 | 524 | 0 | 0 | 524 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 116 | 4 | 0 | 871 | 0 | 875 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 117 | 840 | 0 | 2 | 0 | 842 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 118 | 657 | 0 | 0 | 0 | 657 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 119 | 1 | 758 | 0 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 46 | 1.00 |
| 120 | 0 | 1165 | 0 | 0 | 1165 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 121 | 0 | 962 | 0 | 0 | 962 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 122 | 0 | 906 | 0 | 0 | 906 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 123 | 0 | 759 | 0 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 124 | 849 | 2 | 2 | 0 | 853 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 125 | 0 | 2 | 620 | 0 | 622 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 126 | 0 | 639 | 0 | 0 | 639 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 127 | 3 | 808 | 0 | 0 | 811 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 128 | 639 | 2 | 0 | 0 | 641 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 129 | 547 | 2 | 0 | 0 | 549 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 130 | 2 | 685 | 0 | 0 | 687 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 131 | 2 | 957 | 0 | 0 | 959 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 132 | 792 | 3 | 2 | 0 | 797 | 0.99 | 0.00 | 0.00 | 0.00 | A | 51 | 0.99 |
| 133 | 3 | 0 | 693 | 0 | 696 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 134 | 0 | 0 | 0 | 528 | 528 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 135 | 0 | 0 | 880 | 2 | 882 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 136 | 0 | 0 | 566 | 0 | 566 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 137 | 2 | 534 | 1 | 0 | 537 | 0.00 | 0.99 | 0.00 | 0.00 | T | 51 | 0.99 |
| 138 | 655 | 0 | 0 | 0 | 655 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 139 | 1 | 0 | 1 | 599 | 601 | 0.00 | 0.00 | 0.00 | 1.00 | C | 41 | 1.00 |
| 140 | 0 | 0 | 482 | 0 | 482 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 141 | 0 | 0 | 879 | 0 | 879 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 142 | 0 | 0 | 583 | 0 | 583 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 143 | 0 | 0 | 685 | 0 | 685 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 144 | 0 | 0 | 722 | 0 | 722 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 145 | 0 | 0 | 716 | 2 | 718 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 146 | 0 | 0 | 4 | 536 | 540 | 0.00 | 0.00 | 0.01 | 0.99 | C | 61 | 0.99 |
| 147 | 0 | 0 | 872 | 2 | 874 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 148 | 0 | 0 | 679 | 0 | 679 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 149 | 0 | 378 | 0 | 0 | 378 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 150 | 0 | 4 | 717 | 0 | 721 | 0.00 | 0.01 | 0.99 | 0.00 | G | 51 | 0.99 |
| 151 | 0 | 618 | 2 | 0 | 620 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 152 | 0 | 843 | 0 | 0 | 843 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 153 | 0 | 753 | 0 | 0 | 753 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 154 | 0 | 710 | 0 | 0 | 710 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 155 | 0 | 3 | 1 | 782 | 786 | 0.00 | 0.00 | 0.00 | 0.99 | C | 58 | 0.99 |
| 156 | 0 | 0 | 431 | 3 | 434 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 157 | 0 | 0 | 792 | 0 | 792 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 158 | 0 | 0 | 559 | 0 | 559 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 159 | 0 | 0 | 679 | 0 | 679 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 160 | 0 | 587 | 0 | 0 | 587 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 161 | 0 | 761 | 0 | 0 | 761 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 162 | 0 | 570 | 0 | 0 | 570 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 163 | 564 | 0 | 0 | 0 | 564 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 164 | 0 | 688 | 0 | 0 | 688 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 165 | 0 | 424 | 0 | 0 | 424 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 166 | 0 | 757 | 2 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 167 | 0 | 0 | 1055 | 0 | 1055 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 168 | 0 | 0 | 387 | 0 | 387 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 169 | 0 | 446 | 1 | 0 | 447 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 170 | 0 | 810 | 3 | 0 | 813 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 171 | 0 | 5 | 981 | 0 | 986 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 172 | 0 | 398 | 2 | 0 | 400 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 173 | 604 | 2 | 0 | 0 | 606 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 174 | 0 | 0 | 330 | 0 | 330 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 175 | 0 | 516 | 1 | 0 | 517 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 176 | 2 | 681 | 0 | 0 | 683 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 177 | 491 | 2 | 0 | 1 | 494 | 0.99 | 0.00 | 0.00 | 0.00 | A | 61 | 0.99 |
| 178 | 2 | 0 | 0 | 625 | 627 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 179 | 0 | 0 | 438 | 2 | 440 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 180 | 2 | 511 | 0 | 0 | 513 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 181 | 643 | 3 | 0 | 0 | 646 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 182 | 2 | 628 | 0 | 0 | 630 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 183 | 0 | 822 | 0 | 0 | 822 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 184 | 0 | 769 | 0 | 0 | 769 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 185 | 0 | 804 | 0 | 0 | 804 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 186 | 0 | 784 | 0 | 0 | 784 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 187 | 0 | 785 | 0 | 0 | 785 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 188 | 0 | 808 | 0 | 0 | 808 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 189 | 0 | 780 | 0 | 0 | 780 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 190 | 8 | 583 | 0 | 0 | 591 | 0.01 | 0.99 | 0.00 | 0.00 | T | 28 | 0.99 |
| 191 | 472 | 0 | 0 | 0 | 472 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 192 | 91 | 0 | 399 | 0 | 490 | 0.19 | 0.00 | 0.81 | 0.00 | G | 32 | 0.81 |
| 193 | 0 | 351 | 139 | 0 | 490 | 0.00 | 0.72 | 0.28 | 0.00 | T | 32 | 0.72 |
| 194 | 0 | 2 | 388 | 0 | 390 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 195 | 0 | 0 | 380 | 2 | 382 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 196 | 0 | 0 | 22 | 350 | 372 | 0.00 | 0.00 | 0.06 | 0.94 | C | 58 | 0.94 |
| 197 | 0 | 0 | 415 | 6 | 421 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 198 | 0 | 315 | 0 | 91 | 406 | 0.00 | 0.78 | 0.00 | 0.22 | T | 34 | 0.78 |
| 199 | 0 | 0 | 1 | 592 | 593 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 200 | 0 | 0 | 306 | 0 | 306 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 201 | 112 | 0 | 503 | 0 | 615 | 0.18 | 0.00 | 0.82 | 0.00 | G | 34 | 0.82 |
| 202 | 544 | 0 | 0 | 3 | 547 | 0.99 | 0.00 | 0.00 | 0.01 | A | 46 | 0.99 |
| 203 | 370 | 0 | 0 | 0 | 370 | 1.00 | 0.00 | 0.00 | 0.00 | A | 32 | 1.00 |
| 204 | 0 | 384 | 0 | 0 | 384 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 205 | 0 | 657 | 15 | 0 | 672 | 0.00 | 0.98 | 0.02 | 0.00 | T | 58 | 0.98 |
| 206 | 0 | 6 | 713 | 0 | 719 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 207 | 14 | 272 | 7 | 0 | 293 | 0.05 | 0.93 | 0.02 | 0.00 | T | 58 | 0.93 |
| 208 | 398 | 0 | 0 | 0 | 398 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 209 | 363 | 0 | 0 | 0 | 363 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 210 | 334 | 0 | 71 | 0 | 405 | 0.82 | 0.00 | 0.18 | 0.00 | A | 34 | 0.82 |
| 211 | 10 | 0 | 412 | 0 | 422 | 0.02 | 0.00 | 0.98 | 0.00 | G | 58 | 0.98 |
| 212 | 28 | 262 | 0 | 0 | 290 | 0.10 | 0.90 | 0.00 | 0.00 | T | 51 | 0.90 |
| 213 | 478 | 7 | 0 | 0 | 485 | 0.99 | 0.01 | 0.00 | 0.00 | A | 46 | 0.99 |
| 214 | 1 | 496 | 0 | 0 | 497 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 215 | 0 | 641 | 0 | 0 | 641 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 216 | 0 | 550 | 24 | 0 | 574 | 0.00 | 0.96 | 0.04 | 0.00 | T | 58 | 0.96 |
| 217 | 0 | 10 | 627 | 0 | 637 | 0.00 | 0.02 | 0.98 | 0.00 | G | 51 | 0.98 |
| 218 | 0 | 297 | 131 | 0 | 428 | 0.00 | 0.69 | 0.31 | 0.00 | T | 32 | 0.69 |
| 219 | 32 | 0 | 607 | 0 | 639 | 0.05 | 0.00 | 0.95 | 0.00 | G | 51 | 0.95 |
| 220 | 700 | 0 | 13 | 0 | 713 | 0.98 | 0.00 | 0.02 | 0.00 | A | 58 | 0.98 |
| 221 | 2 | 122 | 507 | 0 | 631 | 0.00 | 0.19 | 0.80 | 0.00 | G | 34 | 0.80 |
| 222 | 0 | 495 | 0 | 0 | 495 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 223 | 0 | 658 | 0 | 0 | 658 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 224 | 0 | 476 | 0 | 29 | 505 | 0.00 | 0.94 | 0.00 | 0.06 | T | 58 | 0.94 |
| 225 | 0 | 2 | 0 | 634 | 636 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 226 | 0 | 2 | 0 | 444 | 446 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 227 | 0 | 450 | 0 | 43 | 493 | 0.00 | 0.91 | 0.00 | 0.09 | T | 58 | 0.91 |
| 228 | 0 | 2 | 0 | 673 | 675 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 229 | 70 | 0 | 285 | 5 | 360 | 0.19 | 0.00 | 0.79 | 0.01 | G | 32 | 0.79 |
| 230 | 401 | 0 | 0 | 18 | 419 | 0.96 | 0.00 | 0.00 | 0.04 | A | 32 | 0.96 |
| 231 | 1 | 69 | 0 | 341 | 411 | 0.00 | 0.17 | 0.00 | 0.83 | C | 31 | 0.83 |
| 232 | 0 | 540 | 228 | 0 | 768 | 0.00 | 0.70 | 0.30 | 0.00 | T | 26 | 0.70 |
| 233 | 0 | 5 | 601 | 0 | 606 | 0.00 | 0.01 | 0.99 | 0.00 | G | 27 | 0.99 |
| 234 | 0 | 296 | 211 | 0 | 507 | 0.00 | 0.58 | 0.42 | 0.00 | T | 25 | 0.58 |
| 235 | 0 | 1 | 588 | 7 | 596 | 0.00 | 0.00 | 0.99 | 0.01 | G | 28 | 0.99 |
| 236 | 0 | 0 | 3 | 296 | 299 | 0.00 | 0.00 | 0.01 | 0.99 | C | 45 | 0.99 |
| 237 | 0 | 116 | 0 | 560 | 676 | 0.00 | 0.17 | 0.00 | 0.83 | C | 31 | 0.83 |
| 238 | 3 | 576 | 0 | 0 | 579 | 0.01 | 0.99 | 0.00 | 0.00 | T | 46 | 0.99 |
| 239 | 141 | 554 | 0 | 32 | 727 | 0.19 | 0.76 | 0.00 | 0.04 | T | 30 | 0.76 |
| 240 | 31 | 38 | 0 | 616 | 685 | 0.05 | 0.06 | 0.00 | 0.90 | C | 26 | 0.90 |
| 241 | 294 | 404 | 0 | 0 | 698 | 0.42 | 0.58 | 0.00 | 0.00 | T | 17 | 0.58 |
| 242 | 1233 | 1 | 0 | 0 | 1234 | 1.00 | 0.00 | 0.00 | 0.00 | A | 27 | 1.00 |
| 243 | 780 | 0 | 0 | 0 | 780 | 1.00 | 0.00 | 0.00 | 0.00 | A | 36 | 1.00 |
| 244 | 110 | 0 | 0 | 0 | 110 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 244 bp |
| Mean of raw sequence Phred quality scores : | 42.34 |
| Quality report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 204 bp |
| Trimmed sequence start position : | 27 |
| Trimmed sequence end position : | 231 |
| Mean of trimmed sequence Phred quality scores : | 46.75 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 244 bp |
| Mean of raw sequence primary peak ratios : | 0.94 |
| Percentage of non-mixed positions on raw sequence : | 88.52 % |
| Mixed peak report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 244 bp |
| Trimmed sequence start position : | 1 |
| Trimmed sequence end position : | 244 |
| Mean of trimmed sequence primary peak ratios : | 0.94 |
| Percentage of non-mixed positions on trimmed sequence : | 88.52 % |
| Final trimmed sequence information | Values |
|---|---|
| Length : | 205 bp |
| Start position : | 27 |
| End position : | 231 |
| Mean of Phred quality scores : | 46.68 |
| Mean of primary peak ratio : | 0.94 |
| Percentage of non-mixed positions : | 89.27 % |
Sequence of primary peaks:
## GTTTTGCGTTGTGCGAAGGGCGGGTGGGCGGGCCGTTGGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTTCCTCGAC
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 27 | 0 | 2 | 2368 | 0 | 2370 | 0.00 | 0.00 | 1.00 | 0.00 | G | 33 | 1.00 |
| 28 | 2 | 2342 | 2 | 0 | 2346 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 29 | 1619 | 1818 | 0 | 0 | 3437 | 0.47 | 0.53 | 0.00 | 0.00 | T | 17 | 0.53 |
| 30 | 0 | 2202 | 0 | 0 | 2202 | 0.00 | 1.00 | 0.00 | 0.00 | T | 17 | 1.00 |
| 31 | 3 | 1323 | 0 | 580 | 1906 | 0.00 | 0.69 | 0.00 | 0.30 | T | 16 | 0.69 |
| 32 | 0 | 0 | 2518 | 0 | 2518 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 33 | 0 | 0 | 0 | 627 | 627 | 0.00 | 0.00 | 0.00 | 1.00 | C | 34 | 1.00 |
| 34 | 0 | 0 | 931 | 0 | 931 | 0.00 | 0.00 | 1.00 | 0.00 | G | 35 | 1.00 |
| 35 | 0 | 1617 | 0 | 0 | 1617 | 0.00 | 1.00 | 0.00 | 0.00 | T | 34 | 1.00 |
| 36 | 0 | 1901 | 3 | 0 | 1904 | 0.00 | 1.00 | 0.00 | 0.00 | T | 29 | 1.00 |
| 37 | 1579 | 0 | 2296 | 0 | 3875 | 0.41 | 0.00 | 0.59 | 0.00 | G | 24 | 0.59 |
| 38 | 0 | 1177 | 0 | 0 | 1177 | 0.00 | 1.00 | 0.00 | 0.00 | T | 27 | 1.00 |
| 39 | 0 | 1 | 796 | 0 | 797 | 0.00 | 0.00 | 1.00 | 0.00 | G | 16 | 1.00 |
| 40 | 610 | 0 | 0 | 707 | 1317 | 0.46 | 0.00 | 0.00 | 0.54 | C | 16 | 0.54 |
| 41 | 0 | 0 | 786 | 1 | 787 | 0.00 | 0.00 | 1.00 | 0.00 | G | 27 | 1.00 |
| 42 | 712 | 192 | 0 | 0 | 904 | 0.79 | 0.21 | 0.00 | 0.00 | A | 22 | 0.79 |
| 43 | 497 | 0 | 364 | 0 | 861 | 0.58 | 0.00 | 0.42 | 0.00 | A | 14 | 0.58 |
| 44 | 0 | 607 | 871 | 0 | 1478 | 0.00 | 0.41 | 0.59 | 0.00 | G | 12 | 0.59 |
| 45 | 0 | 0 | 2 | 0 | 2 | 0.00 | 0.00 | 1.00 | 0.00 | G | 21 | 1.00 |
| 46 | 0 | 0 | 2 | 0 | 2 | 0.00 | 0.00 | 1.00 | 0.00 | G | 18 | 1.00 |
| 47 | 0 | 349 | 1 | 860 | 1210 | 0.00 | 0.29 | 0.00 | 0.71 | C | 19 | 0.71 |
| 48 | 0 | 2 | 979 | 2 | 983 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 49 | 0 | 130 | 715 | 0 | 845 | 0.00 | 0.15 | 0.85 | 0.00 | G | 35 | 0.85 |
| 50 | 0 | 0 | 757 | 0 | 757 | 0.00 | 0.00 | 1.00 | 0.00 | G | 29 | 1.00 |
| 51 | 0 | 428 | 0 | 0 | 428 | 0.00 | 1.00 | 0.00 | 0.00 | T | 27 | 1.00 |
| 52 | 202 | 0 | 797 | 0 | 999 | 0.20 | 0.00 | 0.80 | 0.00 | G | 26 | 0.80 |
| 53 | 0 | 0 | 696 | 0 | 696 | 0.00 | 0.00 | 1.00 | 0.00 | G | 36 | 1.00 |
| 54 | 0 | 172 | 388 | 0 | 560 | 0.00 | 0.31 | 0.69 | 0.00 | G | 33 | 0.69 |
| 55 | 0 | 129 | 0 | 664 | 793 | 0.00 | 0.16 | 0.00 | 0.84 | C | 36 | 0.84 |
| 56 | 0 | 0 | 730 | 282 | 1012 | 0.00 | 0.00 | 0.72 | 0.28 | G | 25 | 0.72 |
| 57 | 0 | 1 | 741 | 349 | 1091 | 0.00 | 0.00 | 0.68 | 0.32 | G | 15 | 0.68 |
| 58 | 0 | 285 | 304 | 0 | 589 | 0.00 | 0.48 | 0.52 | 0.00 | G | 14 | 0.52 |
| 59 | 0 | 462 | 1 | 467 | 930 | 0.00 | 0.50 | 0.00 | 0.50 | C | 14 | 0.50 |
| 60 | 1 | 0 | 0 | 981 | 982 | 0.00 | 0.00 | 0.00 | 1.00 | C | 26 | 1.00 |
| 61 | 192 | 0 | 489 | 0 | 681 | 0.28 | 0.00 | 0.72 | 0.00 | G | 26 | 0.72 |
| 62 | 0 | 302 | 1 | 148 | 451 | 0.00 | 0.67 | 0.00 | 0.33 | T | 15 | 0.67 |
| 63 | 0 | 1241 | 1 | 1 | 1243 | 0.00 | 1.00 | 0.00 | 0.00 | T | 26 | 1.00 |
| 64 | 444 | 0 | 542 | 0 | 986 | 0.45 | 0.00 | 0.55 | 0.00 | G | 15 | 0.55 |
| 65 | 0 | 187 | 658 | 0 | 845 | 0.00 | 0.22 | 0.78 | 0.00 | G | 25 | 0.78 |
| 66 | 0 | 483 | 226 | 0 | 709 | 0.00 | 0.68 | 0.32 | 0.00 | T | 16 | 0.68 |
| 67 | 0 | 994 | 2 | 337 | 1333 | 0.00 | 0.75 | 0.00 | 0.25 | T | 16 | 0.75 |
| 68 | 0 | 772 | 0 | 0 | 772 | 0.00 | 1.00 | 0.00 | 0.00 | T | 40 | 1.00 |
| 69 | 0 | 416 | 0 | 994 | 1410 | 0.00 | 0.30 | 0.00 | 0.70 | C | 29 | 0.70 |
| 70 | 0 | 0 | 327 | 2 | 329 | 0.00 | 0.00 | 0.99 | 0.01 | G | 25 | 0.99 |
| 71 | 0 | 422 | 0 | 308 | 730 | 0.00 | 0.58 | 0.00 | 0.42 | T | 16 | 0.58 |
| 72 | 1 | 1294 | 0 | 0 | 1295 | 0.00 | 1.00 | 0.00 | 0.00 | T | 26 | 1.00 |
| 73 | 589 | 703 | 0 | 0 | 1292 | 0.46 | 0.54 | 0.00 | 0.00 | T | 15 | 0.54 |
| 74 | 622 | 921 | 1 | 0 | 1544 | 0.40 | 0.60 | 0.00 | 0.00 | T | 15 | 0.60 |
| 75 | 0 | 3 | 1242 | 0 | 1245 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 76 | 46 | 0 | 404 | 0 | 450 | 0.10 | 0.00 | 0.90 | 0.00 | G | 51 | 0.90 |
| 77 | 0 | 0 | 630 | 0 | 630 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 78 | 2 | 0 | 644 | 0 | 646 | 0.00 | 0.00 | 1.00 | 0.00 | G | 29 | 1.00 |
| 79 | 893 | 0 | 0 | 0 | 893 | 1.00 | 0.00 | 0.00 | 0.00 | A | 29 | 1.00 |
| 80 | 1 | 0 | 668 | 0 | 669 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 81 | 1 | 0 | 970 | 0 | 971 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 82 | 0 | 0 | 798 | 0 | 798 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 83 | 0 | 0 | 726 | 0 | 726 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 84 | 0 | 756 | 0 | 0 | 756 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 85 | 0 | 914 | 0 | 0 | 914 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 86 | 0 | 3 | 2 | 1071 | 1076 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 87 | 0 | 0 | 652 | 0 | 652 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 88 | 0 | 0 | 4 | 807 | 811 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 89 | 0 | 0 | 1138 | 0 | 1138 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 90 | 0 | 799 | 3 | 0 | 802 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 91 | 0 | 854 | 0 | 0 | 854 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 92 | 0 | 3 | 1047 | 0 | 1050 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 93 | 0 | 605 | 2 | 0 | 607 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 94 | 0 | 912 | 2 | 0 | 914 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 95 | 0 | 2 | 1004 | 0 | 1006 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 96 | 842 | 1 | 2 | 0 | 845 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 97 | 1 | 696 | 0 | 0 | 697 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 98 | 0 | 958 | 1 | 0 | 959 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 99 | 0 | 4 | 1226 | 0 | 1230 | 0.00 | 0.00 | 1.00 | 0.00 | G | 41 | 1.00 |
| 100 | 0 | 2 | 565 | 0 | 567 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 101 | 0 | 518 | 0 | 0 | 518 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 102 | 0 | 957 | 3 | 0 | 960 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 103 | 0 | 5 | 1344 | 0 | 1349 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 104 | 0 | 530 | 0 | 0 | 530 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 105 | 0 | 2 | 1294 | 0 | 1296 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 106 | 0 | 2 | 675 | 0 | 677 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 107 | 0 | 479 | 0 | 1 | 480 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 108 | 0 | 2 | 0 | 991 | 993 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 109 | 0 | 0 | 659 | 2 | 661 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 110 | 0 | 0 | 757 | 0 | 757 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 111 | 2 | 421 | 1 | 0 | 424 | 0.00 | 0.99 | 0.00 | 0.00 | T | 51 | 0.99 |
| 112 | 733 | 1 | 1 | 0 | 735 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 113 | 0 | 0 | 435 | 0 | 435 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 114 | 0 | 2 | 845 | 0 | 847 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 115 | 0 | 524 | 0 | 0 | 524 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 116 | 4 | 0 | 871 | 0 | 875 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 117 | 840 | 0 | 2 | 0 | 842 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 118 | 657 | 0 | 0 | 0 | 657 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 119 | 1 | 758 | 0 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 46 | 1.00 |
| 120 | 0 | 1165 | 0 | 0 | 1165 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 121 | 0 | 962 | 0 | 0 | 962 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 122 | 0 | 906 | 0 | 0 | 906 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 123 | 0 | 759 | 0 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 124 | 849 | 2 | 2 | 0 | 853 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 125 | 0 | 2 | 620 | 0 | 622 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 126 | 0 | 639 | 0 | 0 | 639 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 127 | 3 | 808 | 0 | 0 | 811 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 128 | 639 | 2 | 0 | 0 | 641 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 129 | 547 | 2 | 0 | 0 | 549 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 130 | 2 | 685 | 0 | 0 | 687 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 131 | 2 | 957 | 0 | 0 | 959 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 132 | 792 | 3 | 2 | 0 | 797 | 0.99 | 0.00 | 0.00 | 0.00 | A | 51 | 0.99 |
| 133 | 3 | 0 | 693 | 0 | 696 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 134 | 0 | 0 | 0 | 528 | 528 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 135 | 0 | 0 | 880 | 2 | 882 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 136 | 0 | 0 | 566 | 0 | 566 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 137 | 2 | 534 | 1 | 0 | 537 | 0.00 | 0.99 | 0.00 | 0.00 | T | 51 | 0.99 |
| 138 | 655 | 0 | 0 | 0 | 655 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 139 | 1 | 0 | 1 | 599 | 601 | 0.00 | 0.00 | 0.00 | 1.00 | C | 41 | 1.00 |
| 140 | 0 | 0 | 482 | 0 | 482 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 141 | 0 | 0 | 879 | 0 | 879 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 142 | 0 | 0 | 583 | 0 | 583 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 143 | 0 | 0 | 685 | 0 | 685 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 144 | 0 | 0 | 722 | 0 | 722 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 145 | 0 | 0 | 716 | 2 | 718 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 146 | 0 | 0 | 4 | 536 | 540 | 0.00 | 0.00 | 0.01 | 0.99 | C | 61 | 0.99 |
| 147 | 0 | 0 | 872 | 2 | 874 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 148 | 0 | 0 | 679 | 0 | 679 | 0.00 | 0.00 | 1.00 | 0.00 | G | 51 | 1.00 |
| 149 | 0 | 378 | 0 | 0 | 378 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 150 | 0 | 4 | 717 | 0 | 721 | 0.00 | 0.01 | 0.99 | 0.00 | G | 51 | 0.99 |
| 151 | 0 | 618 | 2 | 0 | 620 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 152 | 0 | 843 | 0 | 0 | 843 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 153 | 0 | 753 | 0 | 0 | 753 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 154 | 0 | 710 | 0 | 0 | 710 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 155 | 0 | 3 | 1 | 782 | 786 | 0.00 | 0.00 | 0.00 | 0.99 | C | 58 | 0.99 |
| 156 | 0 | 0 | 431 | 3 | 434 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 157 | 0 | 0 | 792 | 0 | 792 | 0.00 | 0.00 | 1.00 | 0.00 | G | 61 | 1.00 |
| 158 | 0 | 0 | 559 | 0 | 559 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 159 | 0 | 0 | 679 | 0 | 679 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 160 | 0 | 587 | 0 | 0 | 587 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 161 | 0 | 761 | 0 | 0 | 761 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 162 | 0 | 570 | 0 | 0 | 570 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 163 | 564 | 0 | 0 | 0 | 564 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 164 | 0 | 688 | 0 | 0 | 688 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 165 | 0 | 424 | 0 | 0 | 424 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 166 | 0 | 757 | 2 | 0 | 759 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 167 | 0 | 0 | 1055 | 0 | 1055 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 168 | 0 | 0 | 387 | 0 | 387 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 169 | 0 | 446 | 1 | 0 | 447 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 170 | 0 | 810 | 3 | 0 | 813 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 171 | 0 | 5 | 981 | 0 | 986 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 172 | 0 | 398 | 2 | 0 | 400 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 173 | 604 | 2 | 0 | 0 | 606 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 174 | 0 | 0 | 330 | 0 | 330 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 175 | 0 | 516 | 1 | 0 | 517 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 176 | 2 | 681 | 0 | 0 | 683 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 177 | 491 | 2 | 0 | 1 | 494 | 0.99 | 0.00 | 0.00 | 0.00 | A | 61 | 0.99 |
| 178 | 2 | 0 | 0 | 625 | 627 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 179 | 0 | 0 | 438 | 2 | 440 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 180 | 2 | 511 | 0 | 0 | 513 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 181 | 643 | 3 | 0 | 0 | 646 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 182 | 2 | 628 | 0 | 0 | 630 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 183 | 0 | 822 | 0 | 0 | 822 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 184 | 0 | 769 | 0 | 0 | 769 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 185 | 0 | 804 | 0 | 0 | 804 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 186 | 0 | 784 | 0 | 0 | 784 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 187 | 0 | 785 | 0 | 0 | 785 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 188 | 0 | 808 | 0 | 0 | 808 | 0.00 | 1.00 | 0.00 | 0.00 | T | 61 | 1.00 |
| 189 | 0 | 780 | 0 | 0 | 780 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 190 | 8 | 583 | 0 | 0 | 591 | 0.01 | 0.99 | 0.00 | 0.00 | T | 28 | 0.99 |
| 191 | 472 | 0 | 0 | 0 | 472 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 192 | 91 | 0 | 399 | 0 | 490 | 0.19 | 0.00 | 0.81 | 0.00 | G | 32 | 0.81 |
| 193 | 0 | 351 | 139 | 0 | 490 | 0.00 | 0.72 | 0.28 | 0.00 | T | 32 | 0.72 |
| 194 | 0 | 2 | 388 | 0 | 390 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 195 | 0 | 0 | 380 | 2 | 382 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 196 | 0 | 0 | 22 | 350 | 372 | 0.00 | 0.00 | 0.06 | 0.94 | C | 58 | 0.94 |
| 197 | 0 | 0 | 415 | 6 | 421 | 0.00 | 0.00 | 0.99 | 0.01 | G | 58 | 0.99 |
| 198 | 0 | 315 | 0 | 91 | 406 | 0.00 | 0.78 | 0.00 | 0.22 | T | 34 | 0.78 |
| 199 | 0 | 0 | 1 | 592 | 593 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 200 | 0 | 0 | 306 | 0 | 306 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 201 | 112 | 0 | 503 | 0 | 615 | 0.18 | 0.00 | 0.82 | 0.00 | G | 34 | 0.82 |
| 202 | 544 | 0 | 0 | 3 | 547 | 0.99 | 0.00 | 0.00 | 0.01 | A | 46 | 0.99 |
| 203 | 370 | 0 | 0 | 0 | 370 | 1.00 | 0.00 | 0.00 | 0.00 | A | 32 | 1.00 |
| 204 | 0 | 384 | 0 | 0 | 384 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 205 | 0 | 657 | 15 | 0 | 672 | 0.00 | 0.98 | 0.02 | 0.00 | T | 58 | 0.98 |
| 206 | 0 | 6 | 713 | 0 | 719 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 207 | 14 | 272 | 7 | 0 | 293 | 0.05 | 0.93 | 0.02 | 0.00 | T | 58 | 0.93 |
| 208 | 398 | 0 | 0 | 0 | 398 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 209 | 363 | 0 | 0 | 0 | 363 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 210 | 334 | 0 | 71 | 0 | 405 | 0.82 | 0.00 | 0.18 | 0.00 | A | 34 | 0.82 |
| 211 | 10 | 0 | 412 | 0 | 422 | 0.02 | 0.00 | 0.98 | 0.00 | G | 58 | 0.98 |
| 212 | 28 | 262 | 0 | 0 | 290 | 0.10 | 0.90 | 0.00 | 0.00 | T | 51 | 0.90 |
| 213 | 478 | 7 | 0 | 0 | 485 | 0.99 | 0.01 | 0.00 | 0.00 | A | 46 | 0.99 |
| 214 | 1 | 496 | 0 | 0 | 497 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 215 | 0 | 641 | 0 | 0 | 641 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 216 | 0 | 550 | 24 | 0 | 574 | 0.00 | 0.96 | 0.04 | 0.00 | T | 58 | 0.96 |
| 217 | 0 | 10 | 627 | 0 | 637 | 0.00 | 0.02 | 0.98 | 0.00 | G | 51 | 0.98 |
| 218 | 0 | 297 | 131 | 0 | 428 | 0.00 | 0.69 | 0.31 | 0.00 | T | 32 | 0.69 |
| 219 | 32 | 0 | 607 | 0 | 639 | 0.05 | 0.00 | 0.95 | 0.00 | G | 51 | 0.95 |
| 220 | 700 | 0 | 13 | 0 | 713 | 0.98 | 0.00 | 0.02 | 0.00 | A | 58 | 0.98 |
| 221 | 2 | 122 | 507 | 0 | 631 | 0.00 | 0.19 | 0.80 | 0.00 | G | 34 | 0.80 |
| 222 | 0 | 495 | 0 | 0 | 495 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 223 | 0 | 658 | 0 | 0 | 658 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 224 | 0 | 476 | 0 | 29 | 505 | 0.00 | 0.94 | 0.00 | 0.06 | T | 58 | 0.94 |
| 225 | 0 | 2 | 0 | 634 | 636 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 226 | 0 | 2 | 0 | 444 | 446 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 227 | 0 | 450 | 0 | 43 | 493 | 0.00 | 0.91 | 0.00 | 0.09 | T | 58 | 0.91 |
| 228 | 0 | 2 | 0 | 673 | 675 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 229 | 70 | 0 | 285 | 5 | 360 | 0.19 | 0.00 | 0.79 | 0.01 | G | 32 | 0.79 |
| 230 | 401 | 0 | 0 | 18 | 419 | 0.96 | 0.00 | 0.00 | 0.04 | A | 32 | 0.96 |
| 231 | 1 | 69 | 0 | 341 | 411 | 0.00 | 0.17 | 0.00 | 0.83 | C | 31 | 0.83 |
Sequence of primary peaks:
Length: 228 bp
## AAATAAATTCCATAGCCCTCTAACCTTAATTACTATCACCCTCTTAGTAGAGGGTTAATTACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAACCCTTTAGTGAGGGTTAATTAA
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1957 | 0 | 0 | 1545 | 3502 | 0.56 | 0.00 | 0.00 | 0.44 | A | 5 | 0.56 |
| 2 | 1737 | 839 | 0 | 483 | 3059 | 0.57 | 0.27 | 0.00 | 0.16 | A | 2 | 0.57 |
| 3 | 957 | 0 | 0 | 474 | 1431 | 0.67 | 0.00 | 0.00 | 0.33 | A | 12 | 0.67 |
| 4 | 0 | 1586 | 0 | 0 | 1586 | 0.00 | 1.00 | 0.00 | 0.00 | T | 10 | 1.00 |
| 5 | 2332 | 1251 | 0 | 0 | 3583 | 0.65 | 0.35 | 0.00 | 0.00 | A | 11 | 0.65 |
| 6 | 2346 | 0 | 0 | 436 | 2782 | 0.84 | 0.00 | 0.00 | 0.16 | A | 11 | 0.84 |
| 7 | 2018 | 0 | 0 | 0 | 2018 | 1.00 | 0.00 | 0.00 | 0.00 | A | 35 | 1.00 |
| 8 | 0 | 1153 | 0 | 0 | 1153 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 9 | 0 | 1656 | 0 | 0 | 1656 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 10 | 0 | 0 | 0 | 1460 | 1460 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 11 | 0 | 0 | 0 | 1115 | 1115 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 12 | 1118 | 0 | 282 | 1 | 1401 | 0.80 | 0.00 | 0.20 | 0.00 | A | 5 | 0.80 |
| 13 | 0 | 328 | 2 | 1 | 331 | 0.00 | 0.99 | 0.01 | 0.00 | T | 19 | 0.99 |
| 14 | 1014 | 0 | 0 | 292 | 1306 | 0.78 | 0.00 | 0.00 | 0.22 | A | 8 | 0.78 |
| 15 | 0 | 1 | 383 | 0 | 384 | 0.00 | 0.00 | 1.00 | 0.00 | G | 9 | 1.00 |
| 16 | 0 | 0 | 1 | 1425 | 1426 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 17 | 0 | 0 | 0 | 1400 | 1400 | 0.00 | 0.00 | 0.00 | 1.00 | C | 47 | 1.00 |
| 18 | 0 | 0 | 0 | 1115 | 1115 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 19 | 2 | 1171 | 0 | 0 | 1173 | 0.00 | 1.00 | 0.00 | 0.00 | T | 25 | 1.00 |
| 20 | 495 | 0 | 0 | 924 | 1419 | 0.35 | 0.00 | 0.00 | 0.65 | C | 9 | 0.65 |
| 21 | 0 | 393 | 4 | 1 | 398 | 0.00 | 0.99 | 0.01 | 0.00 | T | 9 | 0.99 |
| 22 | 1359 | 1 | 0 | 0 | 1360 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 23 | 1106 | 0 | 0 | 0 | 1106 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 24 | 0 | 0 | 0 | 547 | 547 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 25 | 206 | 0 | 0 | 714 | 920 | 0.22 | 0.00 | 0.00 | 0.78 | C | 14 | 0.78 |
| 26 | 0 | 548 | 0 | 1 | 549 | 0.00 | 1.00 | 0.00 | 0.00 | T | 30 | 1.00 |
| 27 | 0 | 241 | 0 | 0 | 241 | 0.00 | 1.00 | 0.00 | 0.00 | T | 11 | 1.00 |
| 28 | 889 | 1 | 0 | 0 | 890 | 1.00 | 0.00 | 0.00 | 0.00 | A | 16 | 1.00 |
| 29 | 869 | 1 | 0 | 188 | 1058 | 0.82 | 0.00 | 0.00 | 0.18 | A | 40 | 0.82 |
| 30 | 0 | 655 | 0 | 0 | 655 | 0.00 | 1.00 | 0.00 | 0.00 | T | 47 | 1.00 |
| 31 | 0 | 1008 | 173 | 0 | 1181 | 0.00 | 0.85 | 0.15 | 0.00 | T | 52 | 0.85 |
| 32 | 1102 | 0 | 0 | 1 | 1103 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 33 | 0 | 1 | 0 | 642 | 643 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 34 | 0 | 559 | 0 | 0 | 559 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 35 | 753 | 0 | 0 | 336 | 1089 | 0.69 | 0.00 | 0.00 | 0.31 | A | 9 | 0.69 |
| 36 | 0 | 467 | 0 | 0 | 467 | 0.00 | 1.00 | 0.00 | 0.00 | T | 9 | 1.00 |
| 37 | 0 | 0 | 0 | 1 | 1 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 38 | 1017 | 0 | 0 | 0 | 1017 | 1.00 | 0.00 | 0.00 | 0.00 | A | 32 | 1.00 |
| 39 | 2 | 0 | 0 | 1218 | 1220 | 0.00 | 0.00 | 0.00 | 1.00 | C | 40 | 1.00 |
| 40 | 206 | 0 | 0 | 741 | 947 | 0.22 | 0.00 | 0.00 | 0.78 | C | 25 | 0.78 |
| 41 | 0 | 1 | 0 | 840 | 841 | 0.00 | 0.00 | 0.00 | 1.00 | C | 25 | 1.00 |
| 42 | 0 | 824 | 0 | 0 | 824 | 0.00 | 1.00 | 0.00 | 0.00 | T | 30 | 1.00 |
| 43 | 0 | 0 | 0 | 256 | 256 | 0.00 | 0.00 | 0.00 | 1.00 | C | 22 | 1.00 |
| 44 | 233 | 1145 | 0 | 0 | 1378 | 0.17 | 0.83 | 0.00 | 0.00 | T | 22 | 0.83 |
| 45 | 581 | 794 | 0 | 0 | 1375 | 0.42 | 0.58 | 0.00 | 0.00 | T | 19 | 0.58 |
| 46 | 1370 | 0 | 0 | 0 | 1370 | 1.00 | 0.00 | 0.00 | 0.00 | A | 20 | 1.00 |
| 47 | 0 | 0 | 318 | 0 | 318 | 0.00 | 0.00 | 1.00 | 0.00 | G | 5 | 1.00 |
| 48 | 0 | 403 | 0 | 0 | 403 | 0.00 | 1.00 | 0.00 | 0.00 | T | 3 | 1.00 |
| 49 | 572 | 519 | 6 | 0 | 1097 | 0.52 | 0.47 | 0.01 | 0.00 | A | 5 | 0.52 |
| 50 | 233 | 2 | 352 | 0 | 587 | 0.40 | 0.00 | 0.60 | 0.00 | G | 20 | 0.60 |
| 51 | 1578 | 0 | 0 | 0 | 1578 | 1.00 | 0.00 | 0.00 | 0.00 | A | 23 | 1.00 |
| 52 | 2 | 0 | 1136 | 694 | 1832 | 0.00 | 0.00 | 0.62 | 0.38 | G | 19 | 0.62 |
| 53 | 0 | 0 | 1308 | 617 | 1925 | 0.00 | 0.00 | 0.68 | 0.32 | G | 21 | 0.68 |
| 54 | 0 | 1 | 845 | 557 | 1403 | 0.00 | 0.00 | 0.60 | 0.40 | G | 11 | 0.60 |
| 55 | 0 | 684 | 0 | 548 | 1232 | 0.00 | 0.56 | 0.00 | 0.44 | T | 10 | 0.56 |
| 56 | 0 | 627 | 501 | 0 | 1128 | 0.00 | 0.56 | 0.44 | 0.00 | T | 19 | 0.56 |
| 57 | 1453 | 0 | 0 | 0 | 1453 | 1.00 | 0.00 | 0.00 | 0.00 | A | 14 | 1.00 |
| 58 | 1297 | 0 | 0 | 0 | 1297 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 59 | 370 | 956 | 0 | 0 | 1326 | 0.28 | 0.72 | 0.00 | 0.00 | T | 40 | 0.72 |
| 60 | 0 | 2626 | 0 | 0 | 2626 | 0.00 | 1.00 | 0.00 | 0.00 | T | 22 | 1.00 |
| 61 | 4900 | 6 | 0 | 295 | 5201 | 0.94 | 0.00 | 0.00 | 0.06 | A | 20 | 0.94 |
| 62 | 151 | 0 | 0 | 437 | 588 | 0.26 | 0.00 | 0.00 | 0.74 | C | 10 | 0.74 |
| 63 | 0 | 0 | 0 | 537 | 537 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 64 | 0 | 0 | 626 | 19 | 645 | 0.00 | 0.00 | 0.97 | 0.03 | G | 45 | 0.97 |
| 65 | 0 | 0 | 0 | 621 | 621 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 66 | 0 | 0 | 0 | 871 | 871 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 67 | 0 | 0 | 0 | 811 | 811 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 68 | 0 | 0 | 0 | 901 | 901 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 69 | 0 | 0 | 0 | 792 | 792 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 70 | 0 | 0 | 0 | 707 | 707 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 71 | 0 | 0 | 711 | 0 | 711 | 0.00 | 0.00 | 1.00 | 0.00 | G | 52 | 1.00 |
| 72 | 0 | 396 | 0 | 0 | 396 | 0.00 | 1.00 | 0.00 | 0.00 | T | 23 | 1.00 |
| 73 | 686 | 0 | 0 | 0 | 686 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 74 | 1 | 0 | 0 | 752 | 753 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 75 | 0 | 0 | 0 | 697 | 697 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 76 | 0 | 0 | 798 | 30 | 828 | 0.00 | 0.00 | 0.96 | 0.04 | G | 52 | 0.96 |
| 77 | 0 | 0 | 0 | 511 | 511 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 78 | 0 | 614 | 0 | 1 | 615 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 79 | 641 | 0 | 0 | 0 | 641 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 80 | 683 | 0 | 0 | 0 | 683 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 81 | 0 | 579 | 0 | 0 | 579 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 82 | 0 | 788 | 0 | 0 | 788 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 83 | 777 | 1 | 0 | 0 | 778 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 84 | 481 | 0 | 0 | 0 | 481 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 85 | 0 | 0 | 0 | 477 | 477 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 86 | 0 | 576 | 0 | 0 | 576 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 87 | 744 | 0 | 0 | 0 | 744 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 88 | 548 | 0 | 0 | 0 | 548 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 89 | 511 | 0 | 0 | 0 | 511 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 90 | 690 | 0 | 0 | 0 | 690 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 91 | 569 | 0 | 0 | 0 | 569 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 92 | 1 | 670 | 0 | 0 | 671 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 93 | 0 | 826 | 0 | 0 | 826 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 94 | 1 | 2 | 0 | 697 | 700 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 95 | 363 | 0 | 0 | 0 | 363 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 96 | 0 | 0 | 0 | 625 | 625 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 97 | 0 | 4 | 0 | 719 | 723 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 98 | 0 | 651 | 0 | 3 | 654 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 99 | 823 | 0 | 0 | 2 | 825 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 100 | 2 | 0 | 0 | 652 | 654 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 101 | 0 | 0 | 8 | 607 | 615 | 0.00 | 0.00 | 0.01 | 0.99 | C | 35 | 0.99 |
| 102 | 0 | 0 | 767 | 3 | 770 | 0.00 | 0.00 | 1.00 | 0.00 | G | 35 | 1.00 |
| 103 | 932 | 0 | 2 | 2 | 936 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 104 | 1 | 0 | 0 | 644 | 645 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 105 | 0 | 0 | 0 | 621 | 621 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 106 | 506 | 0 | 0 | 5 | 511 | 0.99 | 0.00 | 0.00 | 0.01 | A | 52 | 0.99 |
| 107 | 2 | 0 | 0 | 540 | 542 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 108 | 473 | 0 | 0 | 2 | 475 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 109 | 541 | 0 | 0 | 2 | 543 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 110 | 2 | 0 | 0 | 584 | 586 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 111 | 1 | 0 | 0 | 650 | 651 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 112 | 538 | 0 | 0 | 2 | 540 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 113 | 565 | 0 | 0 | 0 | 565 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 114 | 0 | 523 | 0 | 0 | 523 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 115 | 0 | 2 | 0 | 775 | 777 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 116 | 494 | 0 | 0 | 2 | 496 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 117 | 459 | 0 | 0 | 0 | 459 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 118 | 6 | 0 | 0 | 488 | 494 | 0.01 | 0.00 | 0.00 | 0.99 | C | 52 | 0.99 |
| 119 | 537 | 0 | 0 | 2 | 539 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 120 | 496 | 0 | 0 | 2 | 498 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 121 | 0 | 0 | 7 | 554 | 561 | 0.00 | 0.00 | 0.01 | 0.99 | C | 45 | 0.99 |
| 122 | 0 | 0 | 624 | 4 | 628 | 0.00 | 0.00 | 0.99 | 0.01 | G | 45 | 0.99 |
| 123 | 0 | 0 | 22 | 615 | 637 | 0.00 | 0.00 | 0.03 | 0.97 | C | 40 | 0.97 |
| 124 | 3 | 0 | 498 | 2 | 503 | 0.01 | 0.00 | 0.99 | 0.00 | G | 40 | 0.99 |
| 125 | 715 | 0 | 0 | 0 | 715 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 126 | 859 | 0 | 0 | 0 | 859 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 127 | 0 | 0 | 0 | 709 | 709 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 128 | 0 | 0 | 0 | 892 | 892 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 129 | 0 | 0 | 0 | 972 | 972 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 130 | 0 | 5 | 0 | 808 | 813 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 131 | 0 | 756 | 0 | 35 | 791 | 0.00 | 0.96 | 0.00 | 0.04 | T | 52 | 0.96 |
| 132 | 0 | 1 | 0 | 1035 | 1036 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 133 | 0 | 0 | 0 | 876 | 876 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 134 | 0 | 0 | 0 | 812 | 812 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 135 | 1 | 0 | 0 | 689 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 136 | 537 | 0 | 0 | 2 | 539 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 137 | 577 | 0 | 0 | 0 | 577 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 138 | 715 | 0 | 0 | 0 | 715 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 139 | 669 | 0 | 0 | 0 | 669 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 140 | 0 | 0 | 2 | 481 | 483 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 141 | 143 | 0 | 507 | 2 | 652 | 0.22 | 0.00 | 0.78 | 0.00 | G | 28 | 0.78 |
| 142 | 657 | 0 | 2 | 0 | 659 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 143 | 831 | 0 | 0 | 0 | 831 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 144 | 573 | 0 | 0 | 0 | 573 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 145 | 1 | 11 | 0 | 578 | 590 | 0.00 | 0.02 | 0.00 | 0.98 | C | 45 | 0.98 |
| 146 | 0 | 640 | 0 | 0 | 640 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 147 | 863 | 2 | 0 | 0 | 865 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 148 | 639 | 0 | 0 | 0 | 639 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 149 | 0 | 0 | 25 | 514 | 539 | 0.00 | 0.00 | 0.05 | 0.95 | C | 45 | 0.95 |
| 150 | 25 | 0 | 674 | 2 | 701 | 0.04 | 0.00 | 0.96 | 0.00 | G | 40 | 0.96 |
| 151 | 783 | 0 | 2 | 2 | 787 | 0.99 | 0.00 | 0.00 | 0.00 | A | 35 | 0.99 |
| 152 | 2 | 0 | 0 | 722 | 724 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 153 | 0 | 0 | 0 | 856 | 856 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 154 | 0 | 0 | 3 | 860 | 863 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 155 | 0 | 0 | 711 | 28 | 739 | 0.00 | 0.00 | 0.96 | 0.04 | G | 52 | 0.96 |
| 156 | 0 | 0 | 2 | 691 | 693 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 157 | 0 | 0 | 0 | 980 | 980 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 158 | 0 | 0 | 0 | 808 | 808 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 159 | 555 | 0 | 0 | 43 | 598 | 0.93 | 0.00 | 0.00 | 0.07 | A | 52 | 0.93 |
| 160 | 0 | 0 | 0 | 758 | 758 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 161 | 0 | 0 | 0 | 844 | 844 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 162 | 0 | 0 | 0 | 807 | 807 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 163 | 140 | 0 | 667 | 2 | 809 | 0.17 | 0.00 | 0.82 | 0.00 | G | 25 | 0.82 |
| 164 | 957 | 0 | 0 | 2 | 959 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 165 | 1 | 0 | 0 | 679 | 680 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 166 | 0 | 6 | 0 | 791 | 797 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 167 | 0 | 623 | 0 | 57 | 680 | 0.00 | 0.92 | 0.00 | 0.08 | T | 52 | 0.92 |
| 168 | 0 | 1 | 0 | 967 | 968 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 169 | 0 | 0 | 483 | 31 | 514 | 0.00 | 0.00 | 0.94 | 0.06 | G | 52 | 0.94 |
| 170 | 4 | 0 | 0 | 502 | 506 | 0.01 | 0.00 | 0.00 | 0.99 | C | 45 | 0.99 |
| 171 | 568 | 9 | 0 | 0 | 577 | 0.98 | 0.02 | 0.00 | 0.00 | A | 52 | 0.98 |
| 172 | 64 | 534 | 0 | 0 | 598 | 0.11 | 0.89 | 0.00 | 0.00 | T | 52 | 0.89 |
| 173 | 903 | 2 | 0 | 0 | 905 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 174 | 634 | 0 | 0 | 0 | 634 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 175 | 477 | 0 | 0 | 0 | 477 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 176 | 0 | 0 | 7 | 596 | 603 | 0.00 | 0.00 | 0.01 | 0.99 | C | 45 | 0.99 |
| 177 | 0 | 0 | 570 | 27 | 597 | 0.00 | 0.00 | 0.95 | 0.05 | G | 52 | 0.95 |
| 178 | 0 | 0 | 27 | 686 | 713 | 0.00 | 0.00 | 0.04 | 0.96 | C | 52 | 0.96 |
| 179 | 20 | 0 | 534 | 0 | 554 | 0.04 | 0.00 | 0.96 | 0.00 | G | 45 | 0.96 |
| 180 | 718 | 0 | 0 | 0 | 718 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 181 | 0 | 477 | 0 | 0 | 477 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 182 | 791 | 2 | 0 | 0 | 793 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 183 | 602 | 0 | 0 | 2 | 604 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 184 | 0 | 0 | 0 | 769 | 769 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 185 | 0 | 0 | 0 | 955 | 955 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 186 | 0 | 5 | 0 | 795 | 800 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 187 | 0 | 662 | 0 | 47 | 709 | 0.00 | 0.93 | 0.00 | 0.07 | T | 52 | 0.93 |
| 188 | 0 | 23 | 0 | 860 | 883 | 0.00 | 0.03 | 0.00 | 0.97 | C | 52 | 0.97 |
| 189 | 0 | 518 | 0 | 2 | 520 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 190 | 843 | 0 | 0 | 0 | 843 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 191 | 550 | 0 | 0 | 2 | 552 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 192 | 2 | 0 | 0 | 640 | 642 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 193 | 0 | 3 | 0 | 730 | 733 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 194 | 0 | 511 | 0 | 0 | 511 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 195 | 794 | 2 | 0 | 0 | 796 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 196 | 561 | 0 | 0 | 0 | 561 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 197 | 568 | 0 | 0 | 0 | 568 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 198 | 621 | 11 | 0 | 0 | 632 | 0.98 | 0.02 | 0.00 | 0.00 | A | 52 | 0.98 |
| 199 | 4 | 718 | 0 | 0 | 722 | 0.01 | 0.99 | 0.00 | 0.00 | T | 52 | 0.99 |
| 200 | 46 | 808 | 0 | 0 | 854 | 0.05 | 0.95 | 0.00 | 0.00 | T | 52 | 0.95 |
| 201 | 794 | 3 | 0 | 0 | 797 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 202 | 3 | 4 | 0 | 596 | 603 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 203 | 155 | 485 | 0 | 2 | 642 | 0.24 | 0.76 | 0.00 | 0.00 | T | 26 | 0.76 |
| 204 | 835 | 2 | 0 | 0 | 837 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 205 | 596 | 0 | 0 | 0 | 596 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 206 | 441 | 0 | 0 | 97 | 538 | 0.82 | 0.00 | 0.00 | 0.18 | A | 26 | 0.82 |
| 207 | 2 | 0 | 0 | 643 | 645 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 208 | 0 | 0 | 0 | 846 | 846 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 209 | 0 | 22 | 0 | 717 | 739 | 0.00 | 0.03 | 0.00 | 0.97 | C | 52 | 0.97 |
| 210 | 0 | 749 | 0 | 0 | 749 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 211 | 18 | 953 | 0 | 0 | 971 | 0.02 | 0.98 | 0.00 | 0.00 | T | 52 | 0.98 |
| 212 | 96 | 661 | 0 | 0 | 757 | 0.13 | 0.87 | 0.00 | 0.00 | T | 52 | 0.87 |
| 213 | 763 | 0 | 0 | 0 | 763 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 214 | 142 | 4 | 567 | 0 | 713 | 0.20 | 0.01 | 0.80 | 0.00 | G | 23 | 0.80 |
| 215 | 0 | 406 | 313 | 0 | 719 | 0.00 | 0.56 | 0.44 | 0.00 | T | 19 | 0.56 |
| 216 | 41 | 0 | 485 | 0 | 526 | 0.08 | 0.00 | 0.92 | 0.00 | G | 35 | 0.92 |
| 217 | 540 | 0 | 267 | 0 | 807 | 0.67 | 0.00 | 0.33 | 0.00 | A | 19 | 0.67 |
| 218 | 1 | 0 | 807 | 0 | 808 | 0.00 | 0.00 | 1.00 | 0.00 | G | 35 | 1.00 |
| 219 | 0 | 0 | 1037 | 0 | 1037 | 0.00 | 0.00 | 1.00 | 0.00 | G | 45 | 1.00 |
| 220 | 0 | 5 | 685 | 0 | 690 | 0.00 | 0.01 | 0.99 | 0.00 | G | 45 | 0.99 |
| 221 | 0 | 567 | 1 | 0 | 568 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 222 | 242 | 553 | 0 | 0 | 795 | 0.30 | 0.70 | 0.00 | 0.00 | T | 28 | 0.70 |
| 223 | 886 | 2 | 0 | 0 | 888 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 224 | 733 | 170 | 0 | 0 | 903 | 0.81 | 0.19 | 0.00 | 0.00 | A | 28 | 0.81 |
| 225 | 105 | 951 | 0 | 0 | 1056 | 0.10 | 0.90 | 0.00 | 0.00 | T | 23 | 0.90 |
| 226 | 1002 | 1905 | 0 | 0 | 2907 | 0.34 | 0.66 | 0.00 | 0.00 | T | 19 | 0.66 |
| 227 | 3827 | 8 | 0 | 0 | 3835 | 1.00 | 0.00 | 0.00 | 0.00 | A | 20 | 1.00 |
| 228 | 380 | 0 | 0 | 0 | 380 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 228 bp |
| Mean of raw sequence Phred quality scores : | 40.9 |
| Quality report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 206 bp |
| Trimmed sequence start position : | 7 |
| Trimmed sequence end position : | 213 |
| Mean of trimmed sequence Phred quality scores : | 42.58 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 228 bp |
| Mean of raw sequence primary peak ratios : | 0.95 |
| Percentage of non-mixed positions on raw sequence : | 91.23 % |
| Mixed peak report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 220 bp |
| Trimmed sequence start position : | 6 |
| Trimmed sequence end position : | 225 |
| Mean of trimmed sequence primary peak ratios : | 0.96 |
| Percentage of non-mixed positions on trimmed sequence : | 93.18 % |
| Final trimmed sequence information | Values |
|---|---|
| Length : | 207 bp |
| Start position : | 7 |
| End position : | 213 |
| Mean of Phred quality scores : | 42.54 |
| Mean of primary peak ratio : | 0.96 |
| Percentage of non-mixed positions : | 94.2 % |
Sequence of primary peaks:
## ATTCCATAGCCCTCTAACCTTAATTACTATCACCCTCTTAGTAGAGGGTTAATTACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAACCCTTTA
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 | 2018 | 0 | 0 | 0 | 2018 | 1.00 | 0.00 | 0.00 | 0.00 | A | 35 | 1.00 |
| 8 | 0 | 1153 | 0 | 0 | 1153 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 9 | 0 | 1656 | 0 | 0 | 1656 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 10 | 0 | 0 | 0 | 1460 | 1460 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 11 | 0 | 0 | 0 | 1115 | 1115 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 12 | 1118 | 0 | 282 | 1 | 1401 | 0.80 | 0.00 | 0.20 | 0.00 | A | 5 | 0.80 |
| 13 | 0 | 328 | 2 | 1 | 331 | 0.00 | 0.99 | 0.01 | 0.00 | T | 19 | 0.99 |
| 14 | 1014 | 0 | 0 | 292 | 1306 | 0.78 | 0.00 | 0.00 | 0.22 | A | 8 | 0.78 |
| 15 | 0 | 1 | 383 | 0 | 384 | 0.00 | 0.00 | 1.00 | 0.00 | G | 9 | 1.00 |
| 16 | 0 | 0 | 1 | 1425 | 1426 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 17 | 0 | 0 | 0 | 1400 | 1400 | 0.00 | 0.00 | 0.00 | 1.00 | C | 47 | 1.00 |
| 18 | 0 | 0 | 0 | 1115 | 1115 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 19 | 2 | 1171 | 0 | 0 | 1173 | 0.00 | 1.00 | 0.00 | 0.00 | T | 25 | 1.00 |
| 20 | 495 | 0 | 0 | 924 | 1419 | 0.35 | 0.00 | 0.00 | 0.65 | C | 9 | 0.65 |
| 21 | 0 | 393 | 4 | 1 | 398 | 0.00 | 0.99 | 0.01 | 0.00 | T | 9 | 0.99 |
| 22 | 1359 | 1 | 0 | 0 | 1360 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 23 | 1106 | 0 | 0 | 0 | 1106 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 24 | 0 | 0 | 0 | 547 | 547 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 25 | 206 | 0 | 0 | 714 | 920 | 0.22 | 0.00 | 0.00 | 0.78 | C | 14 | 0.78 |
| 26 | 0 | 548 | 0 | 1 | 549 | 0.00 | 1.00 | 0.00 | 0.00 | T | 30 | 1.00 |
| 27 | 0 | 241 | 0 | 0 | 241 | 0.00 | 1.00 | 0.00 | 0.00 | T | 11 | 1.00 |
| 28 | 889 | 1 | 0 | 0 | 890 | 1.00 | 0.00 | 0.00 | 0.00 | A | 16 | 1.00 |
| 29 | 869 | 1 | 0 | 188 | 1058 | 0.82 | 0.00 | 0.00 | 0.18 | A | 40 | 0.82 |
| 30 | 0 | 655 | 0 | 0 | 655 | 0.00 | 1.00 | 0.00 | 0.00 | T | 47 | 1.00 |
| 31 | 0 | 1008 | 173 | 0 | 1181 | 0.00 | 0.85 | 0.15 | 0.00 | T | 52 | 0.85 |
| 32 | 1102 | 0 | 0 | 1 | 1103 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 33 | 0 | 1 | 0 | 642 | 643 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 34 | 0 | 559 | 0 | 0 | 559 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 35 | 753 | 0 | 0 | 336 | 1089 | 0.69 | 0.00 | 0.00 | 0.31 | A | 9 | 0.69 |
| 36 | 0 | 467 | 0 | 0 | 467 | 0.00 | 1.00 | 0.00 | 0.00 | T | 9 | 1.00 |
| 37 | 0 | 0 | 0 | 1 | 1 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 38 | 1017 | 0 | 0 | 0 | 1017 | 1.00 | 0.00 | 0.00 | 0.00 | A | 32 | 1.00 |
| 39 | 2 | 0 | 0 | 1218 | 1220 | 0.00 | 0.00 | 0.00 | 1.00 | C | 40 | 1.00 |
| 40 | 206 | 0 | 0 | 741 | 947 | 0.22 | 0.00 | 0.00 | 0.78 | C | 25 | 0.78 |
| 41 | 0 | 1 | 0 | 840 | 841 | 0.00 | 0.00 | 0.00 | 1.00 | C | 25 | 1.00 |
| 42 | 0 | 824 | 0 | 0 | 824 | 0.00 | 1.00 | 0.00 | 0.00 | T | 30 | 1.00 |
| 43 | 0 | 0 | 0 | 256 | 256 | 0.00 | 0.00 | 0.00 | 1.00 | C | 22 | 1.00 |
| 44 | 233 | 1145 | 0 | 0 | 1378 | 0.17 | 0.83 | 0.00 | 0.00 | T | 22 | 0.83 |
| 45 | 581 | 794 | 0 | 0 | 1375 | 0.42 | 0.58 | 0.00 | 0.00 | T | 19 | 0.58 |
| 46 | 1370 | 0 | 0 | 0 | 1370 | 1.00 | 0.00 | 0.00 | 0.00 | A | 20 | 1.00 |
| 47 | 0 | 0 | 318 | 0 | 318 | 0.00 | 0.00 | 1.00 | 0.00 | G | 5 | 1.00 |
| 48 | 0 | 403 | 0 | 0 | 403 | 0.00 | 1.00 | 0.00 | 0.00 | T | 3 | 1.00 |
| 49 | 572 | 519 | 6 | 0 | 1097 | 0.52 | 0.47 | 0.01 | 0.00 | A | 5 | 0.52 |
| 50 | 233 | 2 | 352 | 0 | 587 | 0.40 | 0.00 | 0.60 | 0.00 | G | 20 | 0.60 |
| 51 | 1578 | 0 | 0 | 0 | 1578 | 1.00 | 0.00 | 0.00 | 0.00 | A | 23 | 1.00 |
| 52 | 2 | 0 | 1136 | 694 | 1832 | 0.00 | 0.00 | 0.62 | 0.38 | G | 19 | 0.62 |
| 53 | 0 | 0 | 1308 | 617 | 1925 | 0.00 | 0.00 | 0.68 | 0.32 | G | 21 | 0.68 |
| 54 | 0 | 1 | 845 | 557 | 1403 | 0.00 | 0.00 | 0.60 | 0.40 | G | 11 | 0.60 |
| 55 | 0 | 684 | 0 | 548 | 1232 | 0.00 | 0.56 | 0.00 | 0.44 | T | 10 | 0.56 |
| 56 | 0 | 627 | 501 | 0 | 1128 | 0.00 | 0.56 | 0.44 | 0.00 | T | 19 | 0.56 |
| 57 | 1453 | 0 | 0 | 0 | 1453 | 1.00 | 0.00 | 0.00 | 0.00 | A | 14 | 1.00 |
| 58 | 1297 | 0 | 0 | 0 | 1297 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 59 | 370 | 956 | 0 | 0 | 1326 | 0.28 | 0.72 | 0.00 | 0.00 | T | 40 | 0.72 |
| 60 | 0 | 2626 | 0 | 0 | 2626 | 0.00 | 1.00 | 0.00 | 0.00 | T | 22 | 1.00 |
| 61 | 4900 | 6 | 0 | 295 | 5201 | 0.94 | 0.00 | 0.00 | 0.06 | A | 20 | 0.94 |
| 62 | 151 | 0 | 0 | 437 | 588 | 0.26 | 0.00 | 0.00 | 0.74 | C | 10 | 0.74 |
| 63 | 0 | 0 | 0 | 537 | 537 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 64 | 0 | 0 | 626 | 19 | 645 | 0.00 | 0.00 | 0.97 | 0.03 | G | 45 | 0.97 |
| 65 | 0 | 0 | 0 | 621 | 621 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 66 | 0 | 0 | 0 | 871 | 871 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 67 | 0 | 0 | 0 | 811 | 811 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 68 | 0 | 0 | 0 | 901 | 901 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 69 | 0 | 0 | 0 | 792 | 792 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 70 | 0 | 0 | 0 | 707 | 707 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 71 | 0 | 0 | 711 | 0 | 711 | 0.00 | 0.00 | 1.00 | 0.00 | G | 52 | 1.00 |
| 72 | 0 | 396 | 0 | 0 | 396 | 0.00 | 1.00 | 0.00 | 0.00 | T | 23 | 1.00 |
| 73 | 686 | 0 | 0 | 0 | 686 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 74 | 1 | 0 | 0 | 752 | 753 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 75 | 0 | 0 | 0 | 697 | 697 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 76 | 0 | 0 | 798 | 30 | 828 | 0.00 | 0.00 | 0.96 | 0.04 | G | 52 | 0.96 |
| 77 | 0 | 0 | 0 | 511 | 511 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 78 | 0 | 614 | 0 | 1 | 615 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 79 | 641 | 0 | 0 | 0 | 641 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 80 | 683 | 0 | 0 | 0 | 683 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 81 | 0 | 579 | 0 | 0 | 579 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 82 | 0 | 788 | 0 | 0 | 788 | 0.00 | 1.00 | 0.00 | 0.00 | T | 55 | 1.00 |
| 83 | 777 | 1 | 0 | 0 | 778 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 84 | 481 | 0 | 0 | 0 | 481 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 85 | 0 | 0 | 0 | 477 | 477 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 86 | 0 | 576 | 0 | 0 | 576 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 87 | 744 | 0 | 0 | 0 | 744 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 88 | 548 | 0 | 0 | 0 | 548 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 89 | 511 | 0 | 0 | 0 | 511 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 90 | 690 | 0 | 0 | 0 | 690 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 91 | 569 | 0 | 0 | 0 | 569 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 92 | 1 | 670 | 0 | 0 | 671 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 93 | 0 | 826 | 0 | 0 | 826 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 94 | 1 | 2 | 0 | 697 | 700 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 95 | 363 | 0 | 0 | 0 | 363 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 96 | 0 | 0 | 0 | 625 | 625 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 97 | 0 | 4 | 0 | 719 | 723 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 98 | 0 | 651 | 0 | 3 | 654 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 99 | 823 | 0 | 0 | 2 | 825 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 100 | 2 | 0 | 0 | 652 | 654 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 101 | 0 | 0 | 8 | 607 | 615 | 0.00 | 0.00 | 0.01 | 0.99 | C | 35 | 0.99 |
| 102 | 0 | 0 | 767 | 3 | 770 | 0.00 | 0.00 | 1.00 | 0.00 | G | 35 | 1.00 |
| 103 | 932 | 0 | 2 | 2 | 936 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 104 | 1 | 0 | 0 | 644 | 645 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 105 | 0 | 0 | 0 | 621 | 621 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 106 | 506 | 0 | 0 | 5 | 511 | 0.99 | 0.00 | 0.00 | 0.01 | A | 52 | 0.99 |
| 107 | 2 | 0 | 0 | 540 | 542 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 108 | 473 | 0 | 0 | 2 | 475 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 109 | 541 | 0 | 0 | 2 | 543 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 110 | 2 | 0 | 0 | 584 | 586 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 111 | 1 | 0 | 0 | 650 | 651 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 112 | 538 | 0 | 0 | 2 | 540 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 113 | 565 | 0 | 0 | 0 | 565 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 114 | 0 | 523 | 0 | 0 | 523 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 115 | 0 | 2 | 0 | 775 | 777 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 116 | 494 | 0 | 0 | 2 | 496 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 117 | 459 | 0 | 0 | 0 | 459 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 118 | 6 | 0 | 0 | 488 | 494 | 0.01 | 0.00 | 0.00 | 0.99 | C | 52 | 0.99 |
| 119 | 537 | 0 | 0 | 2 | 539 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 120 | 496 | 0 | 0 | 2 | 498 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 121 | 0 | 0 | 7 | 554 | 561 | 0.00 | 0.00 | 0.01 | 0.99 | C | 45 | 0.99 |
| 122 | 0 | 0 | 624 | 4 | 628 | 0.00 | 0.00 | 0.99 | 0.01 | G | 45 | 0.99 |
| 123 | 0 | 0 | 22 | 615 | 637 | 0.00 | 0.00 | 0.03 | 0.97 | C | 40 | 0.97 |
| 124 | 3 | 0 | 498 | 2 | 503 | 0.01 | 0.00 | 0.99 | 0.00 | G | 40 | 0.99 |
| 125 | 715 | 0 | 0 | 0 | 715 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 126 | 859 | 0 | 0 | 0 | 859 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 127 | 0 | 0 | 0 | 709 | 709 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 128 | 0 | 0 | 0 | 892 | 892 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 129 | 0 | 0 | 0 | 972 | 972 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 130 | 0 | 5 | 0 | 808 | 813 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 131 | 0 | 756 | 0 | 35 | 791 | 0.00 | 0.96 | 0.00 | 0.04 | T | 52 | 0.96 |
| 132 | 0 | 1 | 0 | 1035 | 1036 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 133 | 0 | 0 | 0 | 876 | 876 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 134 | 0 | 0 | 0 | 812 | 812 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 135 | 1 | 0 | 0 | 689 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 136 | 537 | 0 | 0 | 2 | 539 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 137 | 577 | 0 | 0 | 0 | 577 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 138 | 715 | 0 | 0 | 0 | 715 | 1.00 | 0.00 | 0.00 | 0.00 | A | 55 | 1.00 |
| 139 | 669 | 0 | 0 | 0 | 669 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 140 | 0 | 0 | 2 | 481 | 483 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 141 | 143 | 0 | 507 | 2 | 652 | 0.22 | 0.00 | 0.78 | 0.00 | G | 28 | 0.78 |
| 142 | 657 | 0 | 2 | 0 | 659 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 143 | 831 | 0 | 0 | 0 | 831 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 144 | 573 | 0 | 0 | 0 | 573 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 145 | 1 | 11 | 0 | 578 | 590 | 0.00 | 0.02 | 0.00 | 0.98 | C | 45 | 0.98 |
| 146 | 0 | 640 | 0 | 0 | 640 | 0.00 | 1.00 | 0.00 | 0.00 | T | 45 | 1.00 |
| 147 | 863 | 2 | 0 | 0 | 865 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 148 | 639 | 0 | 0 | 0 | 639 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 149 | 0 | 0 | 25 | 514 | 539 | 0.00 | 0.00 | 0.05 | 0.95 | C | 45 | 0.95 |
| 150 | 25 | 0 | 674 | 2 | 701 | 0.04 | 0.00 | 0.96 | 0.00 | G | 40 | 0.96 |
| 151 | 783 | 0 | 2 | 2 | 787 | 0.99 | 0.00 | 0.00 | 0.00 | A | 35 | 0.99 |
| 152 | 2 | 0 | 0 | 722 | 724 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 153 | 0 | 0 | 0 | 856 | 856 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 154 | 0 | 0 | 3 | 860 | 863 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 155 | 0 | 0 | 711 | 28 | 739 | 0.00 | 0.00 | 0.96 | 0.04 | G | 52 | 0.96 |
| 156 | 0 | 0 | 2 | 691 | 693 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 157 | 0 | 0 | 0 | 980 | 980 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 158 | 0 | 0 | 0 | 808 | 808 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 159 | 555 | 0 | 0 | 43 | 598 | 0.93 | 0.00 | 0.00 | 0.07 | A | 52 | 0.93 |
| 160 | 0 | 0 | 0 | 758 | 758 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 161 | 0 | 0 | 0 | 844 | 844 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 162 | 0 | 0 | 0 | 807 | 807 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 163 | 140 | 0 | 667 | 2 | 809 | 0.17 | 0.00 | 0.82 | 0.00 | G | 25 | 0.82 |
| 164 | 957 | 0 | 0 | 2 | 959 | 1.00 | 0.00 | 0.00 | 0.00 | A | 40 | 1.00 |
| 165 | 1 | 0 | 0 | 679 | 680 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 166 | 0 | 6 | 0 | 791 | 797 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 167 | 0 | 623 | 0 | 57 | 680 | 0.00 | 0.92 | 0.00 | 0.08 | T | 52 | 0.92 |
| 168 | 0 | 1 | 0 | 967 | 968 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 169 | 0 | 0 | 483 | 31 | 514 | 0.00 | 0.00 | 0.94 | 0.06 | G | 52 | 0.94 |
| 170 | 4 | 0 | 0 | 502 | 506 | 0.01 | 0.00 | 0.00 | 0.99 | C | 45 | 0.99 |
| 171 | 568 | 9 | 0 | 0 | 577 | 0.98 | 0.02 | 0.00 | 0.00 | A | 52 | 0.98 |
| 172 | 64 | 534 | 0 | 0 | 598 | 0.11 | 0.89 | 0.00 | 0.00 | T | 52 | 0.89 |
| 173 | 903 | 2 | 0 | 0 | 905 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 174 | 634 | 0 | 0 | 0 | 634 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 175 | 477 | 0 | 0 | 0 | 477 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 176 | 0 | 0 | 7 | 596 | 603 | 0.00 | 0.00 | 0.01 | 0.99 | C | 45 | 0.99 |
| 177 | 0 | 0 | 570 | 27 | 597 | 0.00 | 0.00 | 0.95 | 0.05 | G | 52 | 0.95 |
| 178 | 0 | 0 | 27 | 686 | 713 | 0.00 | 0.00 | 0.04 | 0.96 | C | 52 | 0.96 |
| 179 | 20 | 0 | 534 | 0 | 554 | 0.04 | 0.00 | 0.96 | 0.00 | G | 45 | 0.96 |
| 180 | 718 | 0 | 0 | 0 | 718 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 181 | 0 | 477 | 0 | 0 | 477 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 182 | 791 | 2 | 0 | 0 | 793 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 183 | 602 | 0 | 0 | 2 | 604 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 184 | 0 | 0 | 0 | 769 | 769 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 185 | 0 | 0 | 0 | 955 | 955 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 186 | 0 | 5 | 0 | 795 | 800 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 187 | 0 | 662 | 0 | 47 | 709 | 0.00 | 0.93 | 0.00 | 0.07 | T | 52 | 0.93 |
| 188 | 0 | 23 | 0 | 860 | 883 | 0.00 | 0.03 | 0.00 | 0.97 | C | 52 | 0.97 |
| 189 | 0 | 518 | 0 | 2 | 520 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 190 | 843 | 0 | 0 | 0 | 843 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 191 | 550 | 0 | 0 | 2 | 552 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 192 | 2 | 0 | 0 | 640 | 642 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 193 | 0 | 3 | 0 | 730 | 733 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 194 | 0 | 511 | 0 | 0 | 511 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 195 | 794 | 2 | 0 | 0 | 796 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 196 | 561 | 0 | 0 | 0 | 561 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 197 | 568 | 0 | 0 | 0 | 568 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 198 | 621 | 11 | 0 | 0 | 632 | 0.98 | 0.02 | 0.00 | 0.00 | A | 52 | 0.98 |
| 199 | 4 | 718 | 0 | 0 | 722 | 0.01 | 0.99 | 0.00 | 0.00 | T | 52 | 0.99 |
| 200 | 46 | 808 | 0 | 0 | 854 | 0.05 | 0.95 | 0.00 | 0.00 | T | 52 | 0.95 |
| 201 | 794 | 3 | 0 | 0 | 797 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
| 202 | 3 | 4 | 0 | 596 | 603 | 0.00 | 0.01 | 0.00 | 0.99 | C | 52 | 0.99 |
| 203 | 155 | 485 | 0 | 2 | 642 | 0.24 | 0.76 | 0.00 | 0.00 | T | 26 | 0.76 |
| 204 | 835 | 2 | 0 | 0 | 837 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 205 | 596 | 0 | 0 | 0 | 596 | 1.00 | 0.00 | 0.00 | 0.00 | A | 45 | 1.00 |
| 206 | 441 | 0 | 0 | 97 | 538 | 0.82 | 0.00 | 0.00 | 0.18 | A | 26 | 0.82 |
| 207 | 2 | 0 | 0 | 643 | 645 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 208 | 0 | 0 | 0 | 846 | 846 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 209 | 0 | 22 | 0 | 717 | 739 | 0.00 | 0.03 | 0.00 | 0.97 | C | 52 | 0.97 |
| 210 | 0 | 749 | 0 | 0 | 749 | 0.00 | 1.00 | 0.00 | 0.00 | T | 52 | 1.00 |
| 211 | 18 | 953 | 0 | 0 | 971 | 0.02 | 0.98 | 0.00 | 0.00 | T | 52 | 0.98 |
| 212 | 96 | 661 | 0 | 0 | 757 | 0.13 | 0.87 | 0.00 | 0.00 | T | 52 | 0.87 |
| 213 | 763 | 0 | 0 | 0 | 763 | 1.00 | 0.00 | 0.00 | 0.00 | A | 52 | 1.00 |
Sequencing result #1 is aligned on both sense and antisense bisulfite converted DNA sequences to determine the sequencing direction as forward or reverse.
Trimmed sequence of primary peaks from sequencing result #1 is aligned with bisulfite converted reference DNA sense sequence
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:37 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 182
## # Identity: 179/182 (98.4%)
## # Similarity: NA/182 (NA%)
## # Gaps: 0/182 (0.0%)
## # Score: 337.0359
## #
## #
## #=======================================
##
## P1 17 AGGGCGGGTGGGCGGGCCGT 36
## ||| |||||||||||| |||
## S1 108 AGGTCGGGTGGGCGGGTCGT 127
##
## P1 37 TGGTTTCGTTTTGGGGAGGG 56
## | ||||||||||||||||||
## S1 128 TAGTTTCGTTTTGGGGAGGG 147
##
## P1 57 GTTCGCGTTGTTGATTGGTT 76
## ||||||||||||||||||||
## S1 148 GTTCGCGTTGTTGATTGGTT 167
##
## P1 77 GTGGTCGGTAGGTGAATTTT 96
## ||||||||||||||||||||
## S1 168 GTGGTCGGTAGGTGAATTTT 187
##
## P1 97 TAGTTAATTAGCGGTACGGG 116
## ||||||||||||||||||||
## S1 188 TAGTTAATTAGCGGTACGGG 207
##
## P1 117 GGGCGGTGTTTTCGGGGTTT 136
## ||||||||||||||||||||
## S1 208 GGGCGGTGTTTTCGGGGTTT 227
##
## P1 137 ATTTGGTTGTAGTTACGTAT 156
## ||||||||||||||||||||
## S1 228 ATTTGGTTGTAGTTACGTAT 247
##
## P1 157 TTTTTTTTAGTGGCGTCGGA 176
## ||||||||||||||||||||
## S1 248 TTTTTTTTAGTGGCGTCGGA 267
##
## P1 177 ATTGTAAAGTATTTGTGAGT 196
## ||||||||||||||||||||
## S1 268 ATTGTAAAGTATTTGTGAGT 287
##
## P1 197 TT 198
## ||
## S1 288 TT 289
##
##
## #---------------------------------------
## #---------------------------------------
Trimmed sequence of primary peaks from sequencing result #1 is aligned with bisulfite converted reference DNA antisense sequence
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:37 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 6
## # Identity: 6/6 (100.0%)
## # Similarity: NA/6 (NA%)
## # Gaps: 0/6 (0.0%)
## # Score: 11.89054
## #
## #
## #=======================================
##
## P1 150 TACGTA 155
## ||||||
## S1 96 TACGTA 101
##
##
## #---------------------------------------
## #---------------------------------------
Sequencing result #1 corresponds to forward direction
Sequencing result #2 is aligned on both sense and antisense bisulfite converted DNA sequences to determine the sequencing direction as forward or reverse.
Trimmed sequence of primary peaks from sequencing result #2 is aligned with bisulfite converted reference DNA sense sequence
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:38 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 10
## # Identity: 10/10 (100.0%)
## # Similarity: NA/10 (NA%)
## # Gaps: 0/10 (0.0%)
## # Score: 19.81756
## #
## #
## #=======================================
##
## P1 42 TAGAGGGTTA 51
## ||||||||||
## S1 85 TAGAGGGTTA 94
##
##
## #---------------------------------------
## #---------------------------------------
Trimmed sequence of primary peaks from sequencing result #2 is aligned with bisulfite converted reference DNA antisense sequence
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:38 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 146
## # Identity: 146/146 (100.0%)
## # Similarity: NA/146 (NA%)
## # Gaps: 0/146 (0.0%)
## # Score: 289.336
## #
## #
## #=======================================
##
## P1 55 ACCGCCCCCCGTACCGCTAA 74
## ||||||||||||||||||||
## S1 128 ACCGCCCCCCGTACCGCTAA 147
##
## P1 75 TTAACTAAAAATTCACCTAC 94
## ||||||||||||||||||||
## S1 148 TTAACTAAAAATTCACCTAC 167
##
## P1 95 CGACCACAACCAATCAACAA 114
## ||||||||||||||||||||
## S1 168 CGACCACAACCAATCAACAA 187
##
## P1 115 CGCGAACCCCTCCCCAAAAC 134
## ||||||||||||||||||||
## S1 188 CGCGAACCCCTCCCCAAAAC 207
##
## P1 135 GAAACTAACGACCCGCCCAC 154
## ||||||||||||||||||||
## S1 208 GAAACTAACGACCCGCCCAC 227
##
## P1 155 CCGACCTCGCATAAACGCGA 174
## ||||||||||||||||||||
## S1 228 CCGACCTCGCATAAACGCGA 247
##
## P1 175 TAACCCTCTAACCTAAAATT 194
## ||||||||||||||||||||
## S1 248 TAACCCTCTAACCTAAAATT 267
##
## P1 195 ACTAAA 200
## ||||||
## S1 268 ACTAAA 273
##
##
## #---------------------------------------
## #---------------------------------------
Sequencing result #2 corresponds to reverse direction
To find nucleotides of reference DNA sequence positions on sequencing result, the trimmed sequence of primary peaks from forward sequencing results is aligned with bisulfite converted reference DNA sense sequence.
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:38 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 182
## # Identity: 179/182 (98.4%)
## # Similarity: NA/182 (NA%)
## # Gaps: 0/182 (0.0%)
## # Score: 337.0359
## #
## #
## #=======================================
##
## P1 17 AGGGCGGGTGGGCGGGCCGT 36
## ||| |||||||||||| |||
## S1 108 AGGTCGGGTGGGCGGGTCGT 127
##
## P1 37 TGGTTTCGTTTTGGGGAGGG 56
## | ||||||||||||||||||
## S1 128 TAGTTTCGTTTTGGGGAGGG 147
##
## P1 57 GTTCGCGTTGTTGATTGGTT 76
## ||||||||||||||||||||
## S1 148 GTTCGCGTTGTTGATTGGTT 167
##
## P1 77 GTGGTCGGTAGGTGAATTTT 96
## ||||||||||||||||||||
## S1 168 GTGGTCGGTAGGTGAATTTT 187
##
## P1 97 TAGTTAATTAGCGGTACGGG 116
## ||||||||||||||||||||
## S1 188 TAGTTAATTAGCGGTACGGG 207
##
## P1 117 GGGCGGTGTTTTCGGGGTTT 136
## ||||||||||||||||||||
## S1 208 GGGCGGTGTTTTCGGGGTTT 227
##
## P1 137 ATTTGGTTGTAGTTACGTAT 156
## ||||||||||||||||||||
## S1 228 ATTTGGTTGTAGTTACGTAT 247
##
## P1 157 TTTTTTTTAGTGGCGTCGGA 176
## ||||||||||||||||||||
## S1 248 TTTTTTTTAGTGGCGTCGGA 267
##
## P1 177 ATTGTAAAGTATTTGTGAGT 196
## ||||||||||||||||||||
## S1 268 ATTGTAAAGTATTTGTGAGT 287
##
## P1 197 TT 198
## ||
## S1 288 TT 289
##
##
## #---------------------------------------
## #---------------------------------------
Aligned sequence from forward sequencing result (Pattern P1):
Length: 182 bp
## AGGGCGGGTGGGCGGGCCGTTGGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTT
Aligned sequence from bisulfite converted reference DNA sense sequence (Subject S1):
Length: 182 bp
## AGGTCGGGTGGGCGGGTCGTTAGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTT
To find nucleotides of reference DNA sequence positions on sequencing result, the trimmed sequence of primary peaks from reverse sequencing results is aligned with bisulfite converted reference DNA antisense sequence.
## ########################################
## # Program: Biostrings (version 2.64.1), a Bioconductor package
## # Rundate: Fri Jun 9 17:23:38 2023
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 146
## # Identity: 146/146 (100.0%)
## # Similarity: NA/146 (NA%)
## # Gaps: 0/146 (0.0%)
## # Score: 289.336
## #
## #
## #=======================================
##
## P1 55 ACCGCCCCCCGTACCGCTAA 74
## ||||||||||||||||||||
## S1 128 ACCGCCCCCCGTACCGCTAA 147
##
## P1 75 TTAACTAAAAATTCACCTAC 94
## ||||||||||||||||||||
## S1 148 TTAACTAAAAATTCACCTAC 167
##
## P1 95 CGACCACAACCAATCAACAA 114
## ||||||||||||||||||||
## S1 168 CGACCACAACCAATCAACAA 187
##
## P1 115 CGCGAACCCCTCCCCAAAAC 134
## ||||||||||||||||||||
## S1 188 CGCGAACCCCTCCCCAAAAC 207
##
## P1 135 GAAACTAACGACCCGCCCAC 154
## ||||||||||||||||||||
## S1 208 GAAACTAACGACCCGCCCAC 227
##
## P1 155 CCGACCTCGCATAAACGCGA 174
## ||||||||||||||||||||
## S1 228 CCGACCTCGCATAAACGCGA 247
##
## P1 175 TAACCCTCTAACCTAAAATT 194
## ||||||||||||||||||||
## S1 248 TAACCCTCTAACCTAAAATT 267
##
## P1 195 ACTAAA 200
## ||||||
## S1 268 ACTAAA 273
##
##
## #---------------------------------------
## #---------------------------------------
Aligned sequence from reverse sequencing result (Pattern P1):
Length: 146 bp
## ACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAA
Aligned sequence from bisulfite converted reference DNA antisense sequence (Subject S1):
Length: 146 bp
## ACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAA
| Thresholds | Values |
|---|---|
| Minimum length of the aligned sequence : | 30 bp |
| Minimum identity percentage of alignment : | 75 % |
| Minimum of bisulfite conversion rate mean : | 0.9 |
| Direction | Sequencing | Length | Identity | Mismatch | Indel in DNA reference | Indel in sequencing | Mean of conversion rates | SD of conversion rates | Quality Result |
|---|---|---|---|---|---|---|---|---|---|
| Forward | #1 | 182 | 98.35 | 3 | 0 | 0 | 0.98 | 0.1 | Correct |
| Reverse | #2 | 146 | 100.00 | 0 | 0 | 0 | 1.00 | 0.0 | Correct |
| Positions in trimmed sequencing result | Nucleotides in trimmed sequencing result | Positions in reference DNA sequence | Nucleotides in reference DNA sequence |
|---|---|---|---|
| 20 | G | 111 | T |
| 33 | C | 124 | T |
| 38 | G | 129 | A |
No mismatch positions in reverse alignment.
No insertion/deletion in forward alignment.
No insertion/deletion in reverse alignment.
| C number | Bisulfite conversion rate Forward | Bisulfite conversion rate Reverse |
|---|---|---|
| 20 | NA | 1.00 |
| 21 | NA | 1.00 |
| 22 | NA | 1.00 |
| 23 | NA | 1.00 |
| 24 | NA | 1.00 |
| 25 | NA | 1.00 |
| 26 | NA | 1.00 |
| 27 | NA | 1.00 |
| 28 | NA | 1.00 |
| 29 | NA | 1.00 |
| 30 | NaN | 1.00 |
| 31 | 0.50 | 1.00 |
| 32 | 1.00 | 1.00 |
| 33 | 1.00 | 1.00 |
| 34 | 1.00 | 1.00 |
| 35 | 0.58 | 1.00 |
| 36 | 1.00 | 1.00 |
| 37 | 1.00 | 1.00 |
| 38 | 1.00 | 1.00 |
| 39 | 1.00 | 1.00 |
| 40 | 1.00 | 1.00 |
| 41 | 1.00 | 1.00 |
| 42 | 1.00 | 1.00 |
| 43 | 1.00 | 1.00 |
| 44 | 1.00 | 1.00 |
| 45 | 1.00 | 1.00 |
| 46 | 1.00 | 1.00 |
| 47 | 1.00 | 1.00 |
| 48 | 1.00 | 1.00 |
| 49 | 1.00 | 1.00 |
| 50 | 1.00 | 1.00 |
| 51 | 1.00 | NA |
| 52 | 1.00 | NA |
| 53 | 1.00 | NA |
| 54 | 1.00 | NA |
| 55 | 1.00 | NA |
| 56 | 1.00 | NA |
| 57 | 1.00 | NA |
| 58 | 1.00 | NA |
| 59 | 1.00 | NA |
| 60 | 1.00 | NA |
| 61 | 1.00 | NA |
| 62 | 1.00 | NA |
| 63 | 1.00 | NA |
| 64 | 1.00 | NA |
| 65 | 1.00 | NA |
| 66 | 1.00 | NA |
| 67 | 1.00 | NA |
| 68 | 1.00 | NA |
| 69 | 1.00 | NA |
| 70 | 1.00 | NA |
| 71 | 1.00 | NA |
| 72 | 1.00 | NA |
| 73 | 1.00 | NA |
| 74 | 1.00 | NA |
| 75 | 1.00 | NA |
| C number | Position in reference DNA | Position in sequencing result (trimmed) | Position in sequencing result (raw) | A peak | T peak | G peak | C peak | Bisulfite conversion rate |
|---|---|---|---|---|---|---|---|---|
| 30 | 111 | 20 | 46 | 0 | 0 | 2 | 0 | NaN |
| 31 | 124 | 33 | 59 | 0 | 462 | 1 | 467 | 0.50 |
| 32 | 128 | 37 | 63 | 0 | 1241 | 1 | 1 | 1.00 |
| 33 | 131 | 40 | 66 | 0 | 483 | 226 | 0 | 1.00 |
| 34 | 133 | 42 | 68 | 0 | 772 | 0 | 0 | 1.00 |
| 35 | 136 | 45 | 71 | 0 | 422 | 0 | 308 | 0.58 |
| 36 | 137 | 46 | 72 | 1 | 1294 | 0 | 0 | 1.00 |
| 37 | 138 | 47 | 73 | 589 | 703 | 0 | 0 | 1.00 |
| 38 | 150 | 59 | 85 | 0 | 914 | 0 | 0 | 1.00 |
| 39 | 155 | 64 | 90 | 0 | 799 | 3 | 0 | 1.00 |
| 40 | 158 | 67 | 93 | 0 | 605 | 2 | 0 | 1.00 |
| 41 | 166 | 75 | 101 | 0 | 518 | 0 | 0 | 1.00 |
| 42 | 172 | 81 | 107 | 0 | 479 | 0 | 1 | 1.00 |
| 43 | 176 | 85 | 111 | 2 | 421 | 1 | 0 | 1.00 |
| 44 | 184 | 93 | 119 | 1 | 758 | 0 | 0 | 1.00 |
| 45 | 185 | 94 | 120 | 0 | 1165 | 0 | 0 | 1.00 |
| 46 | 186 | 95 | 121 | 0 | 962 | 0 | 0 | 1.00 |
| 47 | 188 | 97 | 123 | 0 | 759 | 0 | 0 | 1.00 |
| 48 | 191 | 100 | 126 | 0 | 639 | 0 | 0 | 1.00 |
| 49 | 192 | 101 | 127 | 3 | 808 | 0 | 0 | 1.00 |
| 50 | 196 | 105 | 131 | 2 | 957 | 0 | 0 | 1.00 |
| 51 | 216 | 125 | 151 | 0 | 618 | 2 | 0 | 1.00 |
| 52 | 217 | 126 | 152 | 0 | 843 | 0 | 0 | 1.00 |
| 53 | 219 | 128 | 154 | 0 | 710 | 0 | 0 | 1.00 |
| 54 | 225 | 134 | 160 | 0 | 587 | 0 | 0 | 1.00 |
| 55 | 227 | 136 | 162 | 0 | 570 | 0 | 0 | 1.00 |
| 56 | 229 | 138 | 164 | 0 | 688 | 0 | 0 | 1.00 |
| 57 | 230 | 139 | 165 | 0 | 424 | 0 | 0 | 1.00 |
| 58 | 234 | 143 | 169 | 0 | 446 | 1 | 0 | 1.00 |
| 59 | 237 | 146 | 172 | 0 | 398 | 2 | 0 | 1.00 |
| 60 | 240 | 149 | 175 | 0 | 516 | 1 | 0 | 1.00 |
| 61 | 241 | 150 | 176 | 2 | 681 | 0 | 0 | 1.00 |
| 62 | 245 | 154 | 180 | 2 | 511 | 0 | 0 | 1.00 |
| 63 | 247 | 156 | 182 | 2 | 628 | 0 | 0 | 1.00 |
| 64 | 248 | 157 | 183 | 0 | 822 | 0 | 0 | 1.00 |
| 65 | 249 | 158 | 184 | 0 | 769 | 0 | 0 | 1.00 |
| 66 | 250 | 159 | 185 | 0 | 804 | 0 | 0 | 1.00 |
| 67 | 251 | 160 | 186 | 0 | 784 | 0 | 0 | 1.00 |
| 68 | 253 | 162 | 188 | 0 | 808 | 0 | 0 | 1.00 |
| 69 | 255 | 164 | 190 | 8 | 583 | 0 | 0 | 1.00 |
| 70 | 269 | 178 | 204 | 0 | 384 | 0 | 0 | 1.00 |
| 71 | 272 | 181 | 207 | 14 | 272 | 7 | 0 | 1.00 |
| 72 | 277 | 186 | 212 | 28 | 262 | 0 | 0 | 1.00 |
| 73 | 279 | 188 | 214 | 1 | 496 | 0 | 0 | 1.00 |
| 74 | 280 | 189 | 215 | 0 | 641 | 0 | 0 | 1.00 |
| 75 | 287 | 196 | 222 | 0 | 495 | 0 | 0 | 1.00 |
| C number | Position in reference DNA | Position in sequencing result (trimmed) | Position in sequencing result (raw) | A peak | T peak | G peak | C peak | Bisulfite conversion rate |
|---|---|---|---|---|---|---|---|---|
| 50 | 146 | 73 | 79 | 641 | 0 | 0 | 0 | 1 |
| 49 | 150 | 77 | 83 | 777 | 1 | 0 | 0 | 1 |
| 48 | 151 | 78 | 84 | 481 | 0 | 0 | 0 | 1 |
| 47 | 154 | 81 | 87 | 744 | 0 | 0 | 0 | 1 |
| 46 | 156 | 83 | 89 | 511 | 0 | 0 | 0 | 1 |
| 45 | 157 | 84 | 90 | 690 | 0 | 0 | 0 | 1 |
| 44 | 158 | 85 | 91 | 569 | 0 | 0 | 0 | 1 |
| 43 | 166 | 93 | 99 | 823 | 0 | 0 | 2 | 1 |
| 42 | 170 | 97 | 103 | 932 | 0 | 2 | 2 | 1 |
| 41 | 176 | 103 | 109 | 541 | 0 | 0 | 2 | 1 |
| 40 | 184 | 111 | 117 | 459 | 0 | 0 | 0 | 1 |
| 39 | 187 | 114 | 120 | 496 | 0 | 0 | 2 | 1 |
| 38 | 192 | 119 | 125 | 715 | 0 | 0 | 0 | 1 |
| 37 | 204 | 131 | 137 | 577 | 0 | 0 | 0 | 1 |
| 36 | 205 | 132 | 138 | 715 | 0 | 0 | 0 | 1 |
| 35 | 206 | 133 | 139 | 669 | 0 | 0 | 0 | 1 |
| 34 | 209 | 136 | 142 | 657 | 0 | 2 | 0 | 1 |
| 33 | 211 | 138 | 144 | 573 | 0 | 0 | 0 | 1 |
| 32 | 214 | 141 | 147 | 863 | 2 | 0 | 0 | 1 |
| 31 | 218 | 145 | 151 | 783 | 0 | 2 | 2 | 1 |
| 30 | 231 | 158 | 164 | 957 | 0 | 0 | 2 | 1 |
| 29 | 241 | 168 | 174 | 634 | 0 | 0 | 0 | 1 |
| 28 | 247 | 174 | 180 | 718 | 0 | 0 | 0 | 1 |
| 27 | 249 | 176 | 182 | 791 | 2 | 0 | 0 | 1 |
| 26 | 258 | 185 | 191 | 550 | 0 | 0 | 2 | 1 |
| 25 | 262 | 189 | 195 | 794 | 2 | 0 | 0 | 1 |
| 24 | 263 | 190 | 196 | 561 | 0 | 0 | 0 | 1 |
| 23 | 265 | 192 | 198 | 621 | 11 | 0 | 0 | 1 |
| 22 | 268 | 195 | 201 | 794 | 3 | 0 | 0 | 1 |
| 21 | 272 | 199 | 205 | 596 | 0 | 0 | 0 | 1 |
| 20 | 273 | 200 | 206 | 441 | 0 | 0 | 97 | 1 |
The maximum aligned sequence corresponds to the maximum sequence covered by at least one correct sequencing.
Length of max aligned sequence : 221 bp
Coordinates of max aligned sequence :
chr16:68771008-68771228
Maximum aligned sequence (on bisulfite converted sense sequence from the plus strand sequence) :
## TTTAGTAATTTTAGGTTAGAGGGTTATCGCGTTTATGCGAGGTCGGGTGGGCGGGTCGTTAGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTT
| CG number | Chromosome | Start | End | Methylation Percentage Forward | Methylation Percentage Reverse | Methylation Percentage Average | Methylation Percentage SD |
|---|---|---|---|---|---|---|---|
| 5 | chr16 | 68771035 | 68771036 | NA | 96.39 | 96.390 | NA |
| 6 | chr16 | 68771037 | 68771038 | NA | 100.00 | 100.000 | NA |
| 7 | chr16 | 68771045 | 68771046 | NA | 100.00 | 100.000 | NA |
| 8 | chr16 | 68771051 | 68771052 | 71.13 | 82.65 | 76.890 | 8.15 |
| 9 | chr16 | 68771059 | 68771060 | 83.73 | 100.00 | 91.865 | 11.50 |
| 10 | chr16 | 68771064 | 68771065 | 100.00 | 96.42 | 98.210 | 2.53 |
| 11 | chr16 | 68771073 | 68771074 | 70.50 | 78.00 | 74.250 | 5.30 |
| 12 | chr16 | 68771090 | 68771091 | 99.72 | 99.40 | 99.560 | 0.23 |
| 13 | chr16 | 68771092 | 68771093 | 100.00 | 100.00 | 100.000 | 0.00 |
| 14 | chr16 | 68771112 | 68771113 | 99.80 | 100.00 | 99.900 | 0.14 |
| 15 | chr16 | 68771138 | 68771139 | 100.00 | 100.00 | 100.000 | 0.00 |
| 16 | chr16 | 68771143 | 68771144 | 100.00 | 100.00 | 100.000 | 0.00 |
| 17 | chr16 | 68771150 | 68771151 | 100.00 | 100.00 | 100.000 | 0.00 |
| 18 | chr16 | 68771159 | 68771160 | 99.62 | NA | 99.620 | NA |
| 19 | chr16 | 68771182 | 68771183 | 100.00 | NA | 100.000 | NA |
| 20 | chr16 | 68771200 | 68771201 | 100.00 | NA | 100.000 | NA |
| 21 | chr16 | 68771203 | 68771204 | 100.00 | NA | 100.000 | NA |
| CG number | Chromosome | Start | End | C position in reference DNA | C position in sequencing result (trimmed) | C position in sequencing result (raw) | A peak | T peak | G peak | C peak | Methylation Percentage |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8 | chr16 | 68771051 | 68771052 | 112 | 21 | 47 | 0 | 349 | 1 | 860 | 71.13 |
| 9 | chr16 | 68771059 | 68771060 | 120 | 29 | 55 | 0 | 129 | 0 | 664 | 83.73 |
| 10 | chr16 | 68771064 | 68771065 | 125 | 34 | 60 | 1 | 0 | 0 | 981 | 100.00 |
| 11 | chr16 | 68771073 | 68771074 | 134 | 43 | 69 | 0 | 416 | 0 | 994 | 70.50 |
| 12 | chr16 | 68771090 | 68771091 | 151 | 60 | 86 | 0 | 3 | 2 | 1071 | 99.72 |
| 13 | chr16 | 68771092 | 68771093 | 153 | 62 | 88 | 0 | 0 | 4 | 807 | 100.00 |
| 14 | chr16 | 68771112 | 68771113 | 173 | 82 | 108 | 0 | 2 | 0 | 991 | 99.80 |
| 15 | chr16 | 68771138 | 68771139 | 199 | 108 | 134 | 0 | 0 | 0 | 528 | 100.00 |
| 16 | chr16 | 68771143 | 68771144 | 204 | 113 | 139 | 1 | 0 | 1 | 599 | 100.00 |
| 17 | chr16 | 68771150 | 68771151 | 211 | 120 | 146 | 0 | 0 | 4 | 536 | 100.00 |
| 18 | chr16 | 68771159 | 68771160 | 220 | 129 | 155 | 0 | 3 | 1 | 782 | 99.62 |
| 19 | chr16 | 68771182 | 68771183 | 243 | 152 | 178 | 2 | 0 | 0 | 625 | 100.00 |
| 20 | chr16 | 68771200 | 68771201 | 261 | 170 | 196 | 0 | 0 | 22 | 350 | 100.00 |
| 21 | chr16 | 68771203 | 68771204 | 264 | 173 | 199 | 0 | 0 | 1 | 592 | 100.00 |
| CG number | Chromosome | Start | End | G position in reference DNA | G position in sequencing result (trimmed) | G position in sequencing result (raw) | A peak | T peak | G peak | C peak | Methylation Percentage |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 17 | chr16 | 68771150 | 68771151 | 131 | 58 | 64 | 0 | 0 | 626 | 19 | 100.00 |
| 16 | chr16 | 68771143 | 68771144 | 138 | 65 | 71 | 0 | 0 | 711 | 0 | 100.00 |
| 15 | chr16 | 68771138 | 68771139 | 143 | 70 | 76 | 0 | 0 | 798 | 30 | 100.00 |
| 14 | chr16 | 68771112 | 68771113 | 169 | 96 | 102 | 0 | 0 | 767 | 3 | 100.00 |
| 13 | chr16 | 68771092 | 68771093 | 189 | 116 | 122 | 0 | 0 | 624 | 4 | 100.00 |
| 12 | chr16 | 68771090 | 68771091 | 191 | 118 | 124 | 3 | 0 | 498 | 2 | 99.40 |
| 11 | chr16 | 68771073 | 68771074 | 208 | 135 | 141 | 143 | 0 | 507 | 2 | 78.00 |
| 10 | chr16 | 68771064 | 68771065 | 217 | 144 | 150 | 25 | 0 | 674 | 2 | 96.42 |
| 9 | chr16 | 68771059 | 68771060 | 222 | 149 | 155 | 0 | 0 | 711 | 28 | 100.00 |
| 8 | chr16 | 68771051 | 68771052 | 230 | 157 | 163 | 140 | 0 | 667 | 2 | 82.65 |
| 7 | chr16 | 68771045 | 68771046 | 236 | 163 | 169 | 0 | 0 | 483 | 31 | 100.00 |
| 6 | chr16 | 68771037 | 68771038 | 244 | 171 | 177 | 0 | 0 | 570 | 27 | 100.00 |
| 5 | chr16 | 68771035 | 68771036 | 246 | 173 | 179 | 20 | 0 | 534 | 0 | 96.39 |
CDH1_High methylated DNA_3_aligned_DNAref_F.fasta
CDH1_High methylated DNA_3_aligned_DNAref_R.fasta
CDH1_High methylated DNA_3_aligned_max_seq.fasta
CDH1_High methylated DNA_3_aligned_seqresult_F.fasta
CDH1_High methylated DNA_3_aligned_seqresult_R.fasta
CDH1_High methylated DNA_3_chromatogram_1.pdf
CDH1_High methylated DNA_3_chromatogram_2.pdf
CDH1_High methylated DNA_3_bisconv_table.csv
CDH1_High methylated DNA_3_bisconv_table.png
CDH1_High methylated DNA_3_bisconv_table.xlsx
CDH1_High methylated DNA_3_meth_table.csv
CDH1_High methylated DNA_3_meth_table.png
CDH1_High methylated DNA_3_meth_table.xlsx
CDH1_High methylated DNA_3_QC.png
CDH1_High methylated DNA_3_QC_table.csv
CDH1_High methylated DNA_3_QC_table.xlsx
CDH1_High methylated DNA_3_seqtable_1.csv
CDH1_High methylated DNA_3_seqtable_1.xlsx
CDH1_High methylated DNA_3_seqtable_2.csv
CDH1_High methylated DNA_3_seqtable_2.xlsx
CDH1_High methylated DNA_3_seqtable_trimmed_1.csv
CDH1_High methylated DNA_3_seqtable_trimmed_1.xlsx
CDH1_High methylated DNA_3_seqtable_trimmed_2.csv
CDH1_High methylated DNA_3_seqtable_trimmed_2.xlsx
CDH1_High methylated DNA_3_trimmedseq.png
| CG_nb | chromosome | start | end | meth_F | meth_R | meth_avg | meth_sd | collection | group | rep | clone | alg_coord_start | alg_coord_end |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5 | chr16 | 68771035 | 68771036 | NA | 96.39 | 96.390 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 6 | chr16 | 68771037 | 68771038 | NA | 100.00 | 100.000 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 7 | chr16 | 68771045 | 68771046 | NA | 100.00 | 100.000 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 8 | chr16 | 68771051 | 68771052 | 71.13 | 82.65 | 76.890 | 8.15 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 9 | chr16 | 68771059 | 68771060 | 83.73 | 100.00 | 91.865 | 11.50 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 10 | chr16 | 68771064 | 68771065 | 100.00 | 96.42 | 98.210 | 2.53 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 11 | chr16 | 68771073 | 68771074 | 70.50 | 78.00 | 74.250 | 5.30 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 12 | chr16 | 68771090 | 68771091 | 99.72 | 99.40 | 99.560 | 0.23 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 13 | chr16 | 68771092 | 68771093 | 100.00 | 100.00 | 100.000 | 0.00 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 14 | chr16 | 68771112 | 68771113 | 99.80 | 100.00 | 99.900 | 0.14 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 15 | chr16 | 68771138 | 68771139 | 100.00 | 100.00 | 100.000 | 0.00 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 16 | chr16 | 68771143 | 68771144 | 100.00 | 100.00 | 100.000 | 0.00 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 17 | chr16 | 68771150 | 68771151 | 100.00 | 100.00 | 100.000 | 0.00 | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 18 | chr16 | 68771159 | 68771160 | 99.62 | NA | 99.620 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 19 | chr16 | 68771182 | 68771183 | 100.00 | NA | 100.000 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 20 | chr16 | 68771200 | 68771201 | 100.00 | NA | 100.000 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 | |
| 21 | chr16 | 68771203 | 68771204 | 100.00 | NA | 100.000 | NA | High methylated DNA | 3 | NA | 68771008 | 68771228 |